Anti-TRMT2A/HTF9C Rabbit Monoclonal Antibody for WB, ELISA - Q8IZ69

Core Product Specifications and Parameters

Biological Background: TRMT2A Function and Localization

• TRMT2A (tRNA methyltransferase 2 homolog A), also known as HTF9C or mRNA (uracil-5-)-methyltransferase, is a member of the methyltransferase superfamily that modifies both tRNAs and mRNAs.
• It catalyzes the S-adenosyl-L-methionine-dependent formation of 5-methyluridine (m5U) at position 54 in cytosolic tRNAs, a modification important for tRNA stability and translation (PubMed:31361898, PubMed:33799331). Related references: PMID:31361898 PMID:33799331
• TRMT2A also mediates m5U modification in certain mRNAs, suggesting a broader role in post-transcriptional regulation (PubMed:34123281). Related references: PMID:34123281
• The protein localizes predominantly to the cytoplasm and cytosol, consistent with its role in processing mature tRNAs and mRNAs.
• Alternative splicing produces multiple isoforms; the canonical isoform has a calculated molecular mass of approximately 68.7 kDa (UniProt: Q8IZ69).
• TRMT2A contains coiled-coil domains and is a reported phosphoprotein, indicating potential regulation by phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1–100 amino acids, a region present in all known isoforms. The antibody may detect multiple splice variants; validate in your specific sample system.
• For Western blot, a band near 69 kDa is expected. Use human cell lines (e.g., HeLa, HEK293) as positive controls and empirically optimize antibody dilution and exposure conditions.
• For ELISA, test the antibody in direct or indirect formats. Performance can be confirmed using recombinant TRMT2A protein or peptide competition assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and life science researchers with streamlined access to IVD raw materials, technical services, and consulting—from concept to clinic. For TRMT2A-related projects, we offer validated antibody pairs, monoclonal/polyclonal antibodies, and bulk ancillary reagents, along with support for rare target sourcing. Our integrated supply model helps simplify your development workflow.