Anti-TrKC Rabbit Monoclonal Antibody for WB, ELISA - Q16288

Core Product Specifications and Parameters

Biological Background: TrKC Function and Localization

• TrKC (also designated NTRK3 or GP145-TrkC) is a member of the neurotrophic tyrosine kinase receptor family and functions as a high-affinity receptor for neurotrophin-3 (NTF3).
• Upon ligand binding, TrKC autophosphorylates and triggers key intracellular signaling cascades, notably the PI3K/AKT and MAPK pathways, which govern cell survival and differentiation.
• Beyond neuronal development, TrKC signaling is implicated in heart development, highlighting broader developmental roles.
• The protein is a single-pass type I transmembrane receptor, with an extracellular region containing immunoglobulin-like domains and leucine-rich repeats, and a cytoplasmic tyrosine kinase domain.
• TrKC is widely expressed but is predominantly found in nervous tissue; isoform 2 exhibits higher expression in adult brain compared to fetal brain.
• Alternative splicing produces multiple isoforms, potentially modulating ligand specificity and signaling output.
• The mature glycosylated protein has a molecular weight of approximately 94 kDa, consistent with a 94428 Da precursor.
• Structural and functional features are annotated with keywords including glycoprotein, kinase, transmembrane, and developmental protein.

Experimental Guidance and Technical Tips

• The immunogen was designed against a synthetic peptide encompassing amino acids 1–100 at the N-terminal extracellular region of human TrKC; this region is likely to detect full-length receptor and certain isoforms.
• For Western blotting, a band around 94 kDa is expected; consider using neural or neuroblastoma cell lines as positive controls and validate with appropriate loading controls.
• Cross-reactivity with mouse and rat is indicated; experimental conditions may need optimization for each species.
• For ELISA applications, consider pairing this antibody with a detection antibody targeting a non-overlapping epitope for sandwich assay development.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner that bridges diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio. This anti-TrKC antibody supports neuroscience and cell signaling research, aligning with our one-stop access model for validated antibodies, bulk ancillary reagents, and custom procurement of rare targets. We streamline the journey from concept to clinic with reliable sourcing and flexible supply solutions.