Anti-TrkB Rabbit Polyclonal Antibody for WB, ELISA - Q16620

Core Product Specifications and Parameters

Biological Background: TrkB Function and Localization

• TrkB (also known as GP145-TrkB, Tropomyosin-related kinase B) is a receptor tyrosine kinase encoded by the NTRK2 gene, playing a critical role in nervous system development and maturation.
• TrkB serves as the high-affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NTF4), and can also bind neurotrophin-3 (NTF3) with lower efficiency to regulate neuron survival (PubMed:15494731, PubMed:7574684). Related references: PMID:15494731, PMID:7574684
• Ligand binding induces receptor homodimerization and autophosphorylation, initiating multiple downstream signaling cascades including the MAPK/ERK and PI3K/AKT pathways (PubMed:15494731). Related references: PMID:15494731
• TrkB activates the GRB2-Ras-MAPK cascade via adaptors like SHC1 and FRS2, promoting neuronal differentiation and neurite outgrowth.
• Through the PI3K-AKT pathway, TrkB supports neuronal growth and survival, and can suppress anoikis (detachment-induced apoptosis).
• TrkB signaling via PLCγ1 activates protein kinase C and NF-κB, regulating synaptic plasticity and transcription of survival genes, thereby contributing to learning and memory.
• Subcellularly, TrkB localizes to the cell membrane, endosomes, axon and dendrite projections, and postsynaptic density, reflecting its roles in signal transduction and trafficking.
• TrkB expression is prominent in the central and peripheral nervous system, including cerebral cortex, hippocampus, and dorsal root ganglia; certain isoforms are also found in pancreas, kidney, and heart.

Experimental Guidance and Technical Tips

• For WB, expect a band around 92 kDa; due to glycosylation (keywords include Glycoprotein), the observed band may migrate slightly higher.
• The immunogen covers the extracellular domain (aa 41-433), making this antibody suitable for detecting both full-length and truncated isoforms in various sample types.
• For ELISA, consider using recombinant TrkB protein as a positive control to validate binding.
• Since cross-reactivity is reported with human and mouse, validate in the relevant sample system; reactivity with other species may need in-house testing.
• As a polyclonal antibody, lot-to-lot variability may occur; consider optimizing dilution and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with comprehensive access to IVD raw materials. For neuroscience and neurotrophin signaling studies, we supply validated TrkB antibodies, recombinant proteins, and ancillary reagents. Contact us for bulk orders or customized sourcing solutions for rare targets.