Anti-TrkA+B+C Monoclonal Antibody for WB, IHC-P, ELISA - P04629 / Q16288 / Q16620

Core Product Specifications and Parameters

Biological Background: TrkA Function and Localization

• TrkA (NTRK1) is a high affinity receptor for nerve growth factor (NGF), playing a central role in the development and maturation of the central and peripheral nervous systems by regulating neuronal proliferation, differentiation, and survival.
  Related references:
  PMID:1281417 PMID:15488758 PMID:17196528
• Multiple isoforms exist: TrkA-I (widely expressed in non-neuronal tissues), TrkA-II (predominantly neuronal), and TrkA-III (specifically in pluripotent neural stem and neural crest progenitors), each with distinct signaling properties.
• Upon NGF binding, TrkA homodimerizes, autophosphorylates, and activates downstream effectors including SHC1, FRS2, and PLCG1, which trigger the Ras-MAPK cascade (cell differentiation), PI3K-AKT pathway (survival), and NF-kappa-B signaling, respectively.
• Isoform TrkA-III is NGF-resistant, constitutively activates AKT1 and NF-kappa-B, antagonizes anti-proliferative NGF-NTRK1 signaling, and promotes angiogenesis and oncogenic activity when overexpressed.
• TrkA localizes to the cell membrane and undergoes ligand-dependent endocytosis, residing in early, late, and recycling endosome membranes, which may sustain or diversify signaling.
• The TrkA receptor is a member of the neurotrophic tyrosine kinase receptor family (also including TrkB/NTRK2 and TrkC/NTRK3); this antibody recognizes a conserved epitope across all three Trk receptors, enabling pan-Trk detection.
• Key functional annotations include: ATP-binding, tyrosine-protein kinase, immunoglobulin domain, leucine-rich repeat, transmembrane helix, glycoprotein, phosphoprotein, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the intracellular region of human NTRK2 (amino acids 722–822), which is conserved across TrkA, TrkB, and TrkC, accounting for pan-Trk reactivity.
• For Western blotting, expect a band around 140 kDa (glycosylated mature form); reducing conditions are typical. Multiple bands or a doublet may appear due to differential glycosylation or cleavage products; validate in your sample system with appropriate controls.
• For immunohistochemistry (paraffin), antigen retrieval (heat-mediated, citrate buffer pH 6.0 or equivalent) is often required; optimize detection based on tissue type and expression levels.
• For ELISA, the antibody can serve as a capture or detection reagent; consider pairing with a different Trk-specific monoclonal antibody or a pan-Trk polyclonal for sandwich assays.
• The antibody is rabbit monoclonal, ensuring high specificity and lot-to-lot consistency; recommended starting dilutions should be determined empirically for each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research laboratories, offering comprehensive IVD raw material sourcing, technical services, and consulting from concept to clinic. This anti-TrkA+B+C monoclonal antibody supports studies in neurotrophin signaling, neurodegenerative diseases, and neural development. Beyond individual products, CamelBio provides validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom raw material sourcing to accelerate your assay development and commercialization.