Anti-TRK Fused Gene (TFG) Rabbit Polyclonal Antibody for WB, ELISA - Q92734

Core Product Specifications and Parameters

Biological Background: TFG Function and Localization

• Protein TFG is a ubiquitously expressed protein localized to the endoplasmic reticulum (ER), where it plays a critical role in ER dynamics and microtubule association (PubMed:23479643, PubMed:27813252). Related references: PMID:23479643 PMID:27813252
• TFG is essential for secretory cargo trafficking from the ER to the Golgi apparatus, ensuring proper protein transport (PubMed:21478858). Related references: PMID:21478858
• The protein is subject to post-translational modifications including acetylation, methylation, and phosphorylation, which may regulate its function and interactions.
• Mutations in TFG are linked to hereditary spastic paraplegia (SPG57), a neurodegenerative disorder, highlighting its critical role in neuronal health.
• As a proto-oncogene, TFG undergoes chromosomal rearrangements that produce oncogenic fusion proteins (e.g., TRK-T3), implicated in certain cancers.
• TFG is predicted to contain coiled-coil domains, which likely mediate protein-protein interactions essential for its cellular functions.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (amino acids 1–240) of TFG, and the antibody may recognize full-length TFG as well as relevant isoforms in Western blotting; a band around 43 kDa is expected.
• For WB analysis, consider using human or mouse cell lysates known to express TFG, and optimize antibody dilution empirically; suitable loading controls should be included.
• In ELISA, this polyclonal antibody can be utilized for direct or indirect detection; validate performance with recombinant TFG protein or biological samples under your specific assay conditions.
• Cross-reactivity with mouse TFG enables its use in murine model studies, but independent validation is recommended for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-TFG polyclonal antibody is part of our portfolio for endoplasmic reticulum and protein trafficking research. We supply validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to support your diagnostic or research needs.