Anti-TRK fused gene (TFG) Rabbit Monoclonal Antibody for WB, ELISA - Q92734

Core Product Specifications and Parameters

Biological Background: TFG Function and Localization

• Protein TFG (TRK-fused gene protein) is a ubiquitously expressed protein encoded by the TFG gene, also known as TF6, HMSNP, SPG57, and TRKT3.
• Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules (PubMed:23479643, PubMed:27813252). Related references: PMID:23479643 PMID:27813252
• Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus (PubMed:21478858). Related references: PMID:21478858
• Subcellularly localized to the endoplasmic reticulum membrane, where it maintains ER morphology and facilitates interactions with the microtubule cytoskeleton.
• Subject to post-translational modifications including phosphorylation, acetylation, and methylation, which may regulate its function in membrane trafficking.
• Mutations in TFG are associated with autosomal recessive hereditary spastic paraplegia (SPG57) and hereditary motor and sensory neuropathy with proximal dominance (HMSNP), linking it to neurodegenerative diseases.
• Chromosomal rearrangements leading to TFG fusion proteins act as proto-oncogenic drivers in certain cancers, such as anaplastic large cell lymphoma and papillary thyroid carcinoma.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 301–400 of human TFG; confirm detection of the full-length 43 kDa protein under your experimental conditions.
• For Western blot (WB), consider using standard protocols and include positive controls expressing TFG at the expected molecular weight.
• Cross-reactivity with mouse has been reported; validate in murine samples if your study involves mouse models, and optimize antibody dilution accordingly.
• For ELISA, titrate the antibody concentration based on the specific assay format and target abundance in your samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves diagnostic manufacturers, laboratories, and research institutions with a comprehensive one-stop sourcing platform for IVD raw materials, from discovery to commercialization. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for key targets like TFG, supporting research on ER dynamics, protein trafficking, and neurodegenerative disease mechanisms. Reach out to our team for customized sourcing of rare-target raw materials or to discuss your supply chain requirements.