Anti-TRIP4 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q15650

Core Product Specifications and Parameters

Biological Background: TRIP4 Function and Localization

• TRIP4 (Activating signal cointegrator 1) functions as a transcription coactivator, associating with nuclear receptors, transcriptional coactivators (EP300, CREBBP, NCOA1), and basal transcription factors (TBP, TFIIA) to facilitate nuclear receptor-mediated transcription.
  Related references: PMID:10454579 PMID:25219498
• It plays a role in thyroid hormone receptor and estrogen receptor transactivation, and is also involved in androgen receptor transactivation (by similarity).
  Related references: PMID:10454579 PMID:25219498
• Acts as a mediator of transrepression between nuclear receptors and AP1 or NF-kappa-B, and is pivotal in the transactivation of NF-kappa-B, SRF, and AP1.
  Related references: PMID:12077347
• As part of the RQC trigger (RQT) complex, TRIP4 activates the ribosome quality control (RQC) pathway, which degrades nascent peptide chains during problematic translation.
  Related references: PMID:32099016 PMID:32579943 PMID:36302773
• May play a role in the development of the neuromuscular junction and late myogenic differentiation.
  Related references: PMID:26924529
• Subcellular localization includes the nucleus, cytoplasm, cytosol, and centrosome.
• Contains a zinc-finger domain and is subject to post-translational modifications including phosphorylation, acetylation, and ubiquitination; disease associations from keywords include congenital muscular dystrophy and neurodegeneration.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a band at approximately 66 kDa corresponding to full-length TRIP4; validate with appropriate positive controls and consider using protease inhibitors during sample preparation.
• For immunohistochemistry paraffin (IHC-P), optimize antigen retrieval conditions (e.g., heat-induced epitope retrieval) and use appropriate tissue controls known to express TRIP4.
• For immunoprecipitation (IP), the polyclonal nature may enhance capture efficiency; titration of antibody amount is recommended to achieve optimal results.
• For ELISA, ensure the recombinant immunogen matches your target sequence; perform cross-validation with a standard curve.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated polyclonal antibodies against key targets like TRIP4. Whether you require bulk quantities of this antibody, matched antibody pairs, or related recombinant proteins, our supply chain supports your project from early-stage research to clinical assay development. Let CamelBio be your reliable partner for sourcing rare targets and essential ancillary reagents.