Anti-TRIP10 Polyclonal Antibody for WB, IF/ICC, ELISA - Q15642

Core Product Specifications and Parameters

Biological Background: TRIP10 Function and Localization

• Encodes Cdc42-interacting protein 4 (CIP4), also known as TRIP-10, a member of the F-BAR protein family involved in coupling membrane deformation to actin cytoskeleton rearrangement.
• Required for insulin-stimulated translocation of glucose transporter GLUT4 to the plasma membrane, facilitating glucose uptake.
• Binds to phosphoinositides and promotes membrane invagination and tubulation during endocytosis; recruits WASL/N-WASP to activate the Arp2/3 complex, driving actin polymerization.
• Essential for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells.
• May contribute to lysosomal retention of FASLG/FASL, implicating a role in immune regulation.
• Localizes to multiple compartments including cytoplasm, cell cortex, cytoskeleton, lysosome, Golgi apparatus, cell membrane, phagocytic cup, and perinuclear region.
• Broadly expressed across many tissues, including brain, heart, kidney, liver, lung, spleen, and testis, as well as immune-related cells like peripheral blood lymphocytes.
• Contains an SH3 domain and coiled-coil motif; regulated by phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 246–545, spanning the SH3 domain and part of the coiled-coil region, likely enabling recognition of multiple TRIP10 isoforms.
• For western blotting, expect a specific band near 68 kDa; include appropriate controls and validate in relevant human or mouse cell lines.
• In IF/ICC experiments, consider fixation and permeabilization protocols that preserve cytoskeletal structures and membrane-associated signals.
• For ELISA applications, optimize coating antigen and antibody dilutions based on your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this TRIP10 polyclonal antibody as part of a comprehensive portfolio of IVD raw materials, supporting research in cell biology, membrane trafficking, and cancer. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets to streamline your assay development. Leverage our end-to-end support from concept to clinic for your next project.