Anti-TRIM9 Rabbit Polyclonal Antibody for WB, ELISA - Q9C026

Core Product Specifications and Parameters

Biological Background: TRIM9 Function and Localization

• E3 ubiquitin-protein ligase that ubiquitinates itself in cooperation with the E2 enzyme UBE2D2/UBC4, targeting itself for proteasomal degradation.
• May play a role in the regulation of neuronal functions and has been implicated in the formation or breakdown of abnormal inclusions in neurodegenerative disorders.
• Acts as a potential regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for SNARE complex formation.
• Alternative splicing produces isoforms; the protein contains characteristic tripartite motif (TRIM) domains including a RING finger, B-box, and coiled-coil region.
• Subcellular localization includes the cytoplasm, dendrites, synaptic vesicles, and cytoskeleton, reflecting its role at neuronal projections and synapses.
• Tissue specificity: predominantly expressed in the brain, with high levels in the cerebral cortex; protein levels are severely decreased in affected brain areas in Parkinson disease and dementia with Lewy bodies.
• Keywords highlight its structural features (zinc-finger, coiled coil) and functional classifications (transferase, Ubl conjugation pathway, phosphoprotein).
• Post-translational modifications include phosphorylation and autoubiquitination, modulating its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-160), which contains the RING and B-box domains; this may produce an antibody that recognizes multiple isoforms and potential ubiquitinated forms.
• For Western blot, a band around 79 kDa is expected based on the theoretical molecular weight; additional bands may appear due to post-translational modifications or degradation—validate with appropriate controls.
• Recommended sample types include brain tissue lysates from human, mouse, or rat, given the high expression in cerebral cortex. For ELISA, consider detecting recombinant or endogenous TRIM9.
• This polyclonal antibody may exhibit batch-to-batch variability inherent to polyclonal production; it is advisable to validate each lot in the specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers and researchers, offering one-stop access to validated IVD raw materials. For TRIM9 and other neuroscience-related targets, we supply validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents. Whether you need standard catalog products or rare-target raw-material sourcing, CamelBio supports your entire workflow from concept to clinical application.