Anti-TRIM72 Rabbit Polyclonal Antibody for WB, ELISA - Q1XH17

Core Product Specifications and Parameters

Biological Background: TRIM72 Function and Localization

• TRIM72, also known as Mitsugumin-53 (MG53), is a muscle-specific E3 ubiquitin-protein ligase that mediates ubiquitination via UBE2D1, UBE2D2, and UBE2D3 E2 enzymes. Related references: PMID:19043407 PMID:37770719
• Acts as an oxidation sensor: upon membrane damage, oxidative environment induces disulfide bond formation, leading to homooligomerization at the injury site, which nucleates the assembly of the repair machinery. Related references: PMID:19043407 PMID:19202355
• Essential for transport of dysferlin (DYSF) to sites of cell injury, facilitating repair patch formation. Related references: PMID:19043407
• Regulates membrane budding and exocytosis, contributing to vesicle trafficking dynamics. Related references: PMID:19029292
• May regulate the mobility of KCNB1-containing endocytic vesicles, influencing potassium channel localization. Related references: PMID:19202355
• Localizes to the cell membrane, particularly the sarcolemma, and to cytoplasmic vesicle membranes, consistent with its role in membrane repair and vesicle trafficking.
• Expressed exclusively in cardiac and skeletal muscle, highlighting its specialized function in muscle tissue homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 200-300 of mouse TRIM72. For WB, a band at ~53 kDa is expected; consider using mouse muscle tissue lysates as positive control.
• For ELISA, the peptide immunogen can be employed as a coating antigen. Validate the antibody in human cell lines or tissues to confirm cross-reactivity.
• Optimal dilutions should be determined empirically in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies, ancillary reagents, and rare target solutions. This anti-TRIM72 antibody supports muscle biology and membrane repair research, complementing our portfolio of high-quality immunological reagents for diagnostic and life science applications.