Anti-TRIM66 Rabbit Polyclonal Antibody for WB - O15016

Core Product Specifications and Parameters

Biological Background: TRIM66 Function and Localization

• Tripartite motif-containing protein 66 (TRIM66), also designated TIF1D, TIF1DELTA, or C11orf29, belongs to the TRIM protein family defined by its tripartite motif (RING, B-box, and coiled-coil domains).
• It is a nuclear protein that may act as a transcription repressor, with its repressive effects mediated at least partly through recruitment of deacetylase activity.
• TRIM66 may function as a negative regulator of postmeiotic genes via complex formation with CBX3 and centromere association (By similarity).
• The protein contains a bromodomain, which typically recognizes acetylated lysine residues on histones, implicating it in chromatin-mediated transcriptional control.
• Multiple zinc-finger motifs and a coiled-coil region are present, suggesting roles in DNA binding and protein-protein interactions.
• Alternative splicing results in multiple TRIM66 isoforms, potentially diversifying its functional repertoire.
• The predicted molecular weight is approximately 149 kDa, reflecting its multi-domain architecture.
• Keywords include metal-binding and zinc, highlighting the structural importance of zinc-binding domains.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 750-970 of human TRIM66; consider its specificity towards particular isoforms or functional regions.
• For Western blot (WB) analysis, use whole-cell or nuclear extracts from human cell lines expressing TRIM66 and confirm a band near 150 kDa.
• In ELISA experiments, titrate the antibody and optimize coating conditions for reproducible results.
• Validate cross-reactivity in your experimental system since the antibody is reported to react with human TRIM66.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated access to IVD raw materials, including a wide range of antibodies for epigenetic and nuclear targets. This anti-TRIM66 antibody supports studies on transcription regulation and chromatin biology, and we can supply validated antibody pairs, optimized polyclonal reagents, and bulk ancillary materials to accelerate your assay development. Our sourcing expertise also extends to rare targets, ensuring you have the critical reagents needed from concept to clinic.