Anti-TRIM62 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BVG3

Core Product Specifications and Parameters

Biological Background: TRIM62 Function and Localization

• TRIM62 (Tripartite motif-containing protein 62), also designated DEAR1, is a member of the TRIM family of E3 ubiquitin ligases, characterized by zinc finger, B-box, and coiled-coil motifs.
• It catalyzes 'Lys-27'-linked ubiquitination of the adaptor CARD9, which activates NF-κB and p38 MAP kinase signaling downstream of C-type lectin receptors, thus promoting antifungal immune responses. Related references: PMID:26488816 PMID:23402750
• Subcellularly, TRIM62 localizes to the cytoplasm.
• The protein is annotated with multiple functional keywords, including innate immunity, transferase activity, metal-binding, and ubiquitin conjugation pathway.
• Alternative splicing gives rise to multiple isoforms of TRIM62.
• Its molecular weight is approximately 54 kDa (calculated 54,268 Da).

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 120–280 of human TRIM62, a region likely to recognize multiple isoforms; however, users should verify isoform-specific detection in their experimental system.
• For Western blot (WB), start with a dilution range of 1:500–1:2000, and adjust based on endogenous expression levels in your cell line or tissue of interest.
• For immunohistochemistry (IHC-P), antigen retrieval methods (e.g., citrate buffer, pH 6.0) are typically recommended to expose epitopes; optimize staining conditions for your sample type.
• While validated for ELISA, pairing with a suitable detection antibody and calibrating against known standards is advised to establish assay sensitivity.
• Given cross-reactivity with mouse and rat, this antibody can be used for comparative studies across species, but confirm specificity with appropriate controls (e.g., knockdown or knockout samples).
• Store undiluted aliquots at -20°C, avoiding freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material procurement, offering one-stop access to high-quality antibodies like this anti-TRIM62 polyclonal antibody for research on ubiquitin ligase and antifungal immunity. From validated antibody pairs and optimized monoclonals to bulk ancillary reagents and rare target sourcing, we simplify your supply chain from concept to clinic.