Anti-TRIM60 Rabbit Polyclonal Antibody for WB and ELISA - Q495X7

Core Product Specifications and Parameters

Biological Background: TRIM60 Function and Localization

• TRIM60, also known as RING finger protein 129 (RNF129) or RNF33, is a tripartite motif-containing protein.
• It functions as an E3 SUMO-protein ligase that mediates SUMOylation of TAB2, leading to inhibition of NF-κB and MAPK signaling pathways by suppressing the TRAF6/TAB2/TAK1 complex.
• According to UniProt keywords, TRIM60 contains a coiled-coil domain and zinc-finger motifs, indicating metal-binding capabilities and potential protein interaction surfaces.
• As a member of the TRIM protein family, TRIM60 likely possesses a RING finger domain, B-box, and coiled-coil region, consistent with its role as an E3 ligase.
• By negatively regulating the TRAF6/TAB2/TAK1 complex, TRIM60 acts as a modulator of innate immune and inflammatory responses.
• No subcellular localization or tissue-specific expression data are currently available in the UniProt record.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1–300), which includes the RING domain. Given the observed mouse cross-reactivity, this region may be highly conserved across species—consider using this antibody in mouse models.
• For Western blot (WB), the predicted molecular weight is 55 kDa. Optimize gel percentage (e.g., 10% SDS-PAGE) and transfer conditions (e.g., wet transfer, PVDF membrane) for detection.
• For ELISA, validate the antibody with appropriate coating buffers and determine the linear detection range using recombinant TRIM60 or cell lysates known to express the target.
• Confirm reactivity in your sample system (e.g., human or mouse immune cell lines) by including positive and negative controls, as TRIM60 expression may be context-dependent.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a streamlined, one-stop sourcing solution for IVD raw materials. Our anti-TRIM60 rabbit polyclonal antibody supports studies on NF-κB signaling and innate immunity. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your project from concept to clinic.