Anti-TRIM59 Polyclonal Antibody for WB, ELISA - Q8IWR1

Core Product Specifications and Parameters

Biological Background: TRIM59 Function and Localization

• TRIM59 (Tripartite motif-containing protein 59), also referred to as RING finger protein 104 (RNF104) or tumor suppressor TSBF-1, is a member of the TRIM family of E3 ubiquitin ligases involved in diverse cellular processes including development and innate immunity. Related references: PMID:22588174 PMID:30231667
• It functions as a negative regulator of innate immune signaling by suppressing RIG-I-like receptor (RLR)-induced activation of interferon regulatory factors IRF3 and IRF7, as well as NF-κB, through interaction with the adaptor protein ECSIT. Related references: PMID:22588174
• TRIM59 modulates autophagy by regulating BECN1 at both the transcriptional and post-translational levels; it downregulates BECN1 transcription via NF-κB pathway inhibition and controls TRAF6-mediated Lys-63-linked ubiquitination of BECN1, impacting the BECN1-PIK3C3 complex assembly. Related references: PMID:30231667
• Additionally, TRIM59 mediates Lys-48-linked ubiquitination of TRAF6, promoting its proteasomal degradation, and further fine-tunes autophagic responses. Related references: PMID:30231667
• A critical role in early embryonic development has been reported, where TRIM59 regulates F-actin assembly and WASH1 Lys-63-linked ubiquitination from the blastocyst to gastrula stages (By similarity).
• The protein localizes predominantly to the endoplasmic reticulum membrane, consistent with its involvement in membrane-associated signaling and autophagy processes.
• TRIM59 features characteristic TRIM domains, including a RING finger domain, B-box, and coiled-coil region, and is annotated with keywords such as zinc-finger, metal-binding, and ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–330 of human TRIM59, encompassing the RING finger and B-box domains, which are well conserved; polyclonal antibodies raised against this region may recognize multiple epitopes and are suitable for detecting both native and denatured forms.
• For Western blotting, expect a band around 47 kDa; validate specificity using positive (e.g., HEK293, HeLa) and negative control lysates, and consider using blocking agents to minimize non-specific binding.
• In ELISA applications, the antibody can be employed as a capture or detection reagent; optimize coating concentrations and cross-reactivity testing with related TRIM family members is advised.
• Given the cross-reactivity with mouse and rat, this antibody is appropriate for translational studies; confirm performance in your specific tissue or cell line of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material provider, offering one-stop access to high-quality antibodies, antigens, and technical services for diagnostic development. The TRIM59 polyclonal antibody is part of our expanding catalog for research in ubiquitination, autophagy, and immune signaling pathways. We support your projects with bulk supply, custom sourcing, and expert consultation to accelerate your assay development from concept to clinic.