Anti-TRIM45 Rabbit Polyclonal Antibody for IF/ICC and ELISA - Q9H8W5

Core Product Specifications and Parameters

Biological Background: TRIM45 Function and Localization

• TRIM45, also known as RNF99, is an E3 ubiquitin-protein ligase that belongs to the tripartite motif (TRIM) family, featuring a RING finger domain, B-box, and coiled-coil region. It is involved in the regulation of inflammatory responses.
• TRIM45 mediates 'Lys-48'-linked polyubiquitination of TAB2, a regulatory protein of the kinase TAK1, targeting it for proteasomal degradation. This process inhibits TLR-mediated inflammatory immune signaling.
  Related references: PMID:36681779
• May also function as a transcriptional repressor in the mitogen-activated protein kinase (MAPK) signaling pathway.
• Localizes to both the cytoplasm and nucleus, consistent with its roles in signal transduction and transcriptional regulation.
• Expressed in multiple tissues, including skeletal muscle, brain, heart, and pancreas.
• Exhibits alternative splicing, producing multiple isoforms that may have distinct functional properties.
• The protein contains zinc-finger motifs (including the RING domain), a coiled-coil region, and undergoes post-translational modifications such as ubiquitination, which are critical for its ligase activity and substrate interaction.
• With a predicted molecular weight of approximately 64 kDa (64359 Da), TRIM45 is subject to proteasomal degradation itself and is identified in proteomic studies.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against a recombinant protein covering amino acids 100–400 of human TRIM45, a region likely encompassing functional domains; it can be used for immunofluorescence/immunocytochemistry (IF/ICC) and ELISA applications.
• For IF/ICC, optimize fixation and permeabilization protocols to detect TRIM45 in both cytoplasmic and nuclear compartments. Consider using appropriate isotype controls.
• In ELISA, validate the antibody as a capture or detection reagent in the specific sample matrix (e.g., cell lysates, tissue extracts) to ensure sensitivity and specificity.
• The antibody cross-reacts with mouse TRIM45, enabling its use in mouse model studies; however, verify cross-reactivity in your experimental setup.
• As a polyclonal antibody, lot-to-lot variability may occur; new lots should be validated for consistency in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with comprehensive access to IVD raw materials, from validated antibody pairs and optimized polyclonal antibodies to bulk ancillary reagents. With expertise in rare target sourcing, we support your research in inflammatory signaling and ubiquitination pathways, helping you secure high-quality TRIM45 antibodies and related tools to accelerate your assay development projects.