Anti-TRIM44 Rabbit Polyclonal Antibody for WB, ELISA - Q96DX7

Core Product Specifications and Parameters

Biological Background: TRIM44 Function and Localization

• TRIM44 (Tripartite motif-containing protein 44), also known as DIPB, is a member of the TRIM (tripartite motif) protein family, characterized by a RING finger domain, B-box type zinc fingers, and a coiled-coil region (predicted from sequence) – though TRIM44 lacks the classical RING domain, it retains the B-box and coiled-coil motifs.
• Encoded by the TRIM44 gene located on human chromosome 2 (Gene ID: 54765), the canonical isoform has a molecular weight of ~38 kDa and is annotated with zinc-finger and metal-binding capabilities, suggesting involvement in protein-protein interactions and possibly ubiquitin-related pathways.
• TRIM44 is implicated in neuronal differentiation and maturation processes, potentially by modulating the activity of other TRIM proteins such as TRIM17 (By similarity).
• TRIM44 functions as a negative regulator of the transcription factor PAX6, a key factor in eye, brain, and pancreatic development, thereby influencing gene expression programs critical for cellular identity. Related references: PMID:26394807
• Consistent with a role in developmental regulation, TRIM44 is highly expressed in testis, indicating potential functions in spermatogenesis or germ cell maintenance.
• Post-translational modifications include phosphorylation (as evidenced by phosphoprotein keyword), which may regulate its activity or localization in response to cellular signals.
• Clinically, TRIM44 has been associated with disease variants (keyword: Disease variant), though specific pathogenic mutations await further characterization, highlighting its potential relevance in human pathology.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody corresponds to amino acids 205-344 of human TRIM44, a region likely spanning the coiled-coil and C-terminal domains; for optimal detection, consider validating in sample systems with confirmed TRIM44 expression (e.g., testis or neuronal cells).
• In Western blot (WB) applications, a band at approximately 38 kDa is expected; due to potential post-translational modifications or isoform variation, additional bands may appear – consider using appropriate controls and molecular weight markers.
• For ELISA, titrate the antibody and optimize coating antigen concentrations; cross-species reactivity with mouse and rat TRIM44 (based on sequence homology) enables multi-species studies, but verify binding affinity in your specific assay conditions.
• As a polyclonal antibody, batch-to-batch variability is inherent; include internal reference standards and perform routine quality checks to ensure consistent performance across experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying high-quality IVD raw materials, offering a comprehensive range of antibodies and reagents to support diagnostic development from concept to market. For researchers exploring TRIM44-related pathways in neurodevelopment, testicular biology, or cancer, we provide validated polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents tailored to your assay needs. Whether you require rare target sourcing or large-scale manufacturing support, CamelBio acts as a reliable bridge between your scientific requirements and global supply chains.