Anti-TRIM31 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BZY9

Core Product Specifications and Parameters

Biological Background: TRIM31 Function and Localization

• TRIM31, also known as tripartite motif-containing protein 31, is an E3 ubiquitin-protein ligase belonging to the TRIM/RBCC family, characterized by a RING finger domain, B-box, and coiled-coil region.
• It functions as a key regulator of innate antiviral immunity by mediating Lys-63-linked ubiquitination of mitochondrial antiviral signaling protein (MAVS), promoting MAVS polymerization and filament formation on mitochondria to activate downstream signaling. Related references: PMID:27992402
• TRIM31 negatively regulates the NLRP3 inflammasome by catalyzing Lys-48-linked ubiquitination of NLRP3, leading to its proteasomal degradation and attenuation of inflammatory responses. Related references: PMID:27929086
• It also acts as a regulator of Src-induced anchorage-independent cell growth, suggesting a potential role in tumorigenesis (By similarity).
• The protein localizes to both the cytoplasm and mitochondria, consistent with its dual roles in antiviral signaling and inflammasome regulation.
• TRIM31 expression is upregulated in gastric adenocarcinomas, indicating possible involvement in cancer progression.
• It contains a zinc-finger domain and metal-binding sites, crucial for its E3 ligase activity and protein interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 186-425) of human TRIM31, suggesting the antibody may detect full-length TRIM31 in Western blotting at the predicted molecular weight of ~48 kDa.
• For immunofluorescence/ICC, anticipate cytoplasmic and mitochondrial staining patterns; use appropriate organelle markers to confirm localization.
• This antibody is validated for ELISA; consider its use in capture or detection formats when paired with a suitable matched antibody pair.
• Cross-reactivity with mouse TRIM31 is reported; for mouse samples, verify sequence homology in the immunogen region and consider testing in knockout cell lines if available.
• As a polyclonal antibody, lot-to-lot consistency may vary; include positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For TRIM31 and other innate immunity targets, we provide reliable raw materials to support your diagnostic assay development from concept to clinic. Contact us to explore custom sourcing solutions for rare or challenging targets.