Anti-TRIM25 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q14258

Core Product Specifications and Parameters

Biological Background: TRIM25 Function and Localization

• Encodes an E3 ubiquitin/ISG15 ligase that plays a pivotal role in innate immunity by mediating 'Lys-63'-linked polyubiquitination of RIG-I (DDX58) and IFIH1 (MDA5), leading to type I interferon production upon viral infection. Related references: PMID:17392790, PMID:29357390, PMID:30193849

• Functions as an estrogen-responsive protein, mediating estrogen action through ubiquitination and proteasomal degradation of ZFHX3, thereby linking hormonal signaling to protein turnover. Related references: PMID:22452784

• Acts as an ISG15 E3 ligase, promoting ISGylation of 14-3-3 sigma (SFN) and participating in broader ISGylation pathways. Related references: PMID:16352599, PMID:17069755

• Mediates 'Lys-63'-linked polyubiquitination of the antiviral protein ZAP/ZC3HAV1, which is required for optimal binding to target viral mRNA. Related references: PMID:28060952, PMID:28202764

• Promotes the restart of stalled replication forks by interacting with the KHDC3L-OOEP scaffold and ubiquitinating BLM helicase, facilitating BLM recruitment to DNA damage sites.

• Involved in autophagy suppression by ubiquitinating and degrading the autophagy receptor p62/SQSTM1. Related references: PMID:40542578

• Ubiquitinates substrates involved in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis, and mRNA translation/stability, as revealed by proteomic studies. Related references: PMID:36067236

• Subcellularly localizes to the cytoplasm, stress granules, and nucleus, reflecting its diverse functional roles. Belongs to the TRIM protein family characterized by a RING finger, B-box zinc-finger(s), and coiled-coil domain.

• Ubiquitously expressed, with notable expression in breast tumors at the protein level.

Experimental Guidance and Technical Tips

• For Western blotting, expect a band near 71 kDa; use appropriate positive controls such as HeLa cell lysates and validate with a blocking peptide or knockdown control.
• In IHC-P, consider optimizing antigen retrieval conditions (e.g., citrate buffer, pH 6.0) and validate with known TRIM25-positive tissues like breast carcinoma.
• For IF/ICC, test fixation/permeabilization protocols (e.g., methanol vs. PFA/Triton) as different localizations (cytoplasm, stress granules, nucleus) may require tailored conditions.
• This polyclonal antibody cross-reacts with mouse and rat; verify species-specific reactivity in your experimental system by comparing sequence homology of the immunogen region.
• For IP, titrate antibody carefully to minimize non-specific binding; include an isotype control and pre-clearing step.
• ELISA applications may benefit from coating with the immunogen fragment; confirm linearity and specificity using recombinant TRIM25 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined procurement channel for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. We specialize in supporting innate immunity and cancer research projects, like those involving TRIM25, by sourcing rare targets and providing technical consulting across the concept-to-clinic continuum. Let us be your trusted partner in accelerating assay development and biomarker validation.