Anti-TRIM25 Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q14258

Core Product Specifications and Parameters

Biological Background: TRIM25 Function and Localization

• TRIM25 (Tripartite motif-containing protein 25), also known as EFP, RNF147, ZNF147, is a member of the TRIM protein family defined by the presence of a RING finger domain, B-box, and coiled-coil region.
• Functions as an E3 ubiquitin and ISG15 ligase essential for innate immune defense, mediating 'Lys-63'-linked polyubiquitination of RIG-I (DDX58) and IFIH1 to trigger interferon production upon viral infection. Related references: PMID:17392790 PMID:29357390 PMID:30193849 PMID:23950712
• Promotes ISGylation of 14-3-3 sigma (SFN), regulating signaling pathways involved in cell cycle and apoptosis. Related references: PMID:16352599 PMID:17069755
• Mediates estrogen action by targeting the transcription factor ZFHX3 for ubiquitination and proteasomal degradation, influencing hormone-dependent gene expression. Related references: PMID:22452784
• Participates in DNA damage response by promoting restart of stalled replication forks via ubiquitination of BLM helicase, in cooperation with the KHDC3L-OOEP scaffold.
• Supports the antiviral activity of ZAP/ZC3HAV1 by catalyzing 'Lys-63'-linked polyubiquitination, which enhances its binding to viral mRNA. Related references: PMID:28060952 PMID:28202764
• Suppresses autophagy through ubiquitination and degradation of the autophagy receptor p62/SQSTM1. Related references: PMID:40542578
• Found in the cytoplasm, stress granules, and nucleus, and is ubiquitously expressed with elevated levels observed in breast tumors.

Experimental Guidance and Technical Tips

• For Western blot (WB), expect a specific band around 71 kDa; knockdown validation (KD) may be used to confirm antibody specificity.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and fixation methods; suitable dilutions should be experimentally determined.
• For immunoprecipitation (IP), as a rabbit monoclonal antibody, it provides consistent and high-affinity capture; include appropriate isotype controls.
• This antibody recognizes residues 190–379 of human TRIM25; verify cross-reactivity if using in non-human systems.
• Store according to recommended conditions and avoid repeated freeze-thaw cycles to preserve antibody integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined sourcing solution for IVD developers, offering a comprehensive range of validated antibody pairs, optimized monoclonal antibodies like this TRIM25 clone, and bulk ancillary reagents to support rapid assay development and scale-up. Whether you require ready-to-use antibodies or custom rare-target material sourcing, our platform bridges the gap from concept to clinical application in immunology and infectious disease research.