Anti-TRIM24 Rabbit Monoclonal Antibody for WB, ELISA - O15164

Core Product Specifications and Parameters

Biological Background: TRIM24 Function and Localization

• TRIM24 (Transcription intermediary factor 1-alpha) is a member of the tripartite motif (TRIM) family, also known as TIF1α, RNF82, and PTC6.
• Acts as a transcriptional coactivator that interacts with various nuclear receptors, including androgen receptor (AR), glucocorticoid receptor (GCR/NR3C1), thyroid hormone receptor (TR), estrogen receptor alpha (ESR1), and retinoic acid receptors (RARA), thereby up-regulating ligand-dependent transcription and modulating hepatocyte proliferation (By similarity).
• Interacts with chromatin, demonstrating highest affinity for histone H3 unmodified at Lys-4 (H3K4me0) and acetylated at Lys-23 (H3K23ac), indicating its role in chromatin-mediated transcription regulation.
• Functions as an E3 ubiquitin-protein ligase and participates in the DNA damage response by forming an autoregulatory feedback loop with TP53: ATM kinase phosphorylates TRIM24, leading to its ubiquitination and degradation, while TRIM24 subsequently ubiquitinates TP53 for degradation, thus controlling cell proliferation and apoptosis. Related references: PMID:24820418
• Involved in innate immunity by mediating Lys-63-linked ubiquitination of TRAF3, which activates type I interferon signaling. Related references: PMID:32324863
• Negatively regulates NLRP3 inflammasome-mediated pyroptosis and cell migration, likely through ubiquitination of NLRP3. Related references: PMID:33724611
• Localizes to the nucleus, cytoplasm, and mitochondrion, as indicated by UniProt annotation.
• Undergoes post-translational modifications such as phosphorylation and methylation, and contains structural domains including a bromodomain, zinc finger (RING-type, B-box-type), and coiled-coil region, contributing to its diverse functions.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic peptide corresponding to amino acids 400-500 of human TRIM24 (O15164), a region conserved in mouse and rat, consistent with cross-reactivity data.
• For Western blot (WB), the predicted molecular weight of TRIM24 is 117kDa; consider using appropriate gel percentage and transfer conditions for high molecular weight proteins.
• For ELISA applications, validate the optimal coating concentration and detection range using recombinant TRIM24 or cell lysates from human, mouse, or rat samples.
• Due to TRIM24's multiple subcellular localizations, subcellular fractionation or organelle isolation may be beneficial for studying compartment-specific functions.

CamelBio: Your One-Stop Sourcing Bridge

As a trusted provider of high-quality IVD raw materials, CamelBio supports diagnostic developers and researchers with validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. This anti-TRIM24 rabbit monoclonal antibody is suited for studies in transcriptional regulation, DNA damage signaling, and immuno-oncology. For custom sourcing of rare targets or assay-specific needs, CamelBio delivers tailored procurement solutions to accelerate your R&D pipeline.