Anti-TRIM24 Polyclonal Antibody for WB, ELISA - O15164

Core Product Specifications and Parameters

Biological Background: TRIM24 Function and Localization

• TRIM24 (Transcription intermediary factor 1-alpha) is a member of the tripartite motif (TRIM) family, also known as E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, and TIF1-alpha. It functions as a transcriptional coactivator and an E3 ubiquitin ligase.
• TRIM24 interacts with chromatin by binding histone H3 when it is unmodified at Lys-4 (H3K4me0) and acetylated at Lys-23 (H3K23ac), mediating gene regulation. Its PHD finger and bromodomain recognize these modifications.
• Early in the DNA damage response, ATM kinase phosphorylates TRIM24, leading to its ubiquitination and degradation. After sufficient DNA repair, TP53 activates TRIM24 transcription, and TRIM24 in turn ubiquitinates TP53 for degradation, forming an autoregulatory feedback loop. Related references: PMID:24820418
• TRIM24 plays a role in regulating cell proliferation and apoptosis through its effects on p53/TP53 levels. It up-regulates ligand-dependent transcription activation by nuclear receptors, including androgen receptor (AR), glucocorticoid receptor (GCR/NR3C1), thyroid hormone receptor (TR), estrogen receptor (ESR1), and retinoic acid receptors (RARs).
• In innate immunity, TRIM24 mediates the specific Lys-63-linked ubiquitination of TRAF3, leading to activation of the type I interferon pathway. Related references: PMID:32324863
• TRIM24 negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration, likely by ubiquitinating NLRP3. Related references: PMID:33724611
• TRIM24 localizes to the nucleus, cytoplasm, and mitochondria, reflecting its diverse functional roles.
• Keywords associated with TRIM24 include bromodomain, PHD finger, RING-type zinc finger, coiled coil, transcriptional regulation, tumor suppressor, and protein ubiquitination, highlighting its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 600–820, which includes the PHD finger and bromodomain involved in histone H3 binding. This region is likely to be conserved, so consider the possibility of cross-reactivity with related bromodomain-containing proteins and include appropriate controls.
• For Western blotting, TRIM24 migrates at approximately 117 kDa. To confirm band specificity, include a negative control such as a TRIM24 knockout cell lysate or use siRNA knockdown.
• This antibody is suitable for ELISA. Due to its polyclonal nature, we recommend including a no-primary antibody control and pre-immune serum control when setting up assays.
• Perform titration experiments to determine the optimal dilution. Common starting dilutions for polyclonal antibodies in Western blotting may range from 1:500 to 1:2000. Always validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. This anti-TRIM24 polyclonal antibody is part of our extensive portfolio supporting cancer research, epigenetics, and innate immunity. We also offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare target raw materials to streamline your assay development.