Anti-TRIM23 Polyclonal Antibody for WB, IF/ICC, ELISA - P36406

Core Product Specifications and Parameters

Biological Background: TRIM23 Function and Localization

• TRIM23 (tripartite motif-containing protein 23), also known as ARD1, ARFD1, and RNF46, is a member of the TRIM protein family featuring an N-terminal RING finger domain, coiled-coil segments, and a C-terminal ADP-ribosylation factor (ARF) domain that exhibits GTPase activity.
• TRIM23 is essential for autophagy activation during viral infection. It promotes TANK-binding kinase 1 (TBK1) dimerization, enabling the subsequent phosphorylation of the selective autophagy receptor SQSTM1, a key step in autophagosome formation. TRIM23's own ARF domain undergoes 'Lys-27'-linked auto-ubiquitination to stimulate its GTPase activity and recruitment to autophagosomal membranes. Related references: PMID:31189704, PMID:40360474, PMID:28871090
• Under conditions of cellular energy stress, TRIM23 catalyzes 'Lys-63'-linked polyubiquitination of HAX1, triggering its liquid-liquid phase separation and contributing to the assembly of cytoplasmic processing bodies (P-bodies). Related references: PMID:38769438
• In the context of host-microbe interactions, TRIM23 mediates TRAF6 auto-ubiquitination in the presence of the human cytomegalovirus (HCMV) protein UL144, leading to virally controlled activation of NF-κB signaling.
• Subcellularly, TRIM23 localizes primarily to the cytoplasm, with association to Golgi apparatus and lysosome membranes; it also relocates to P-bodies upon specific stress stimuli.
• Additional post-translational modifications include phosphorylation, and the protein possesses zinc-finger motifs for metal coordination; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 280 amino acids of human TRIM23, suggesting reactivity with the N-terminal region. For western blot, a band around 64 kDa is expected; consider using lysates from human, mouse, or rat cell lines.
• For immunofluorescence, anticipate cytoplasmic and Golgi-associated staining patterns; co-localization studies with organelle markers may further validate subcellular distribution.
• Given the polyclonal nature, lot-to-lot consistency should be verified. Optimize antibody dilution for each application by titration experiments in your sample system.
• When investigating autophagy or ubiquitination pathways, include appropriate controls such as autophagy inhibitors, proteasome inhibitors, or knockdown models to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw-material sourcing, supporting diagnostic manufacturers and researchers from early development through clinical application. This anti-TRIM23 polyclonal antibody, targeting a key player in autophagy, ubiquitination, and innate immunity, is part of our comprehensive portfolio of validated antibodies, antibody pairs, and ancillary reagents. Whether you need bulk quantities, custom development, or rare target raw materials, CamelBio provides reliable, one-stop access to accelerate your assay development.