Anti-TRIM21/SS-A Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P19474

Core Product Specifications and Parameters

Biological Background: TRIM21/SS-A Function and Localization

• TRIM21, also known as Ro52/SS-A, is a 52 kDa ribonucleoprotein autoantigen that exists as a ribonucleoprotein particle composed of a single polypeptide and small RNA molecules. At least two isoforms are present in nucleated and red blood cells, with tissue-specific differences in expression. Related references: PMID:8666824
• Functions as an E3 ubiquitin-protein ligase dependent on E2 enzymes (UBE2D1, UBE2D2, UBE2E1, UBE2E2), mediating self-ubiquitination and ubiquitination of substrates such as USP4 and IKBKB, and forming a complex with UBE2D2. Related references: PMID:16297862, PMID:16316627, PMID:16472766, PMID:16880511, PMID:19675099
• Component of the SCF(SKP2)-like E3 ubiquitin-protein ligase complex, which promotes ubiquitination and proteasomal degradation of CDKN1B/p27 in a phosphorylation-dependent manner, thereby regulating cell cycle progression. Related references: PMID:16880511
• Negatively regulates IFN-beta production by catalyzing polyubiquitin-mediated degradation of IRF3, and modulates NF-kappa-B signaling through monoubiquitination of IKBKB. Related references: PMID:18641315, PMID:19675099
• Serves as a platform for autophagic machinery assembly by recruiting ULK1, Beclin 1, and ATG8 family members, and regulates autophagy initiation and FIP200 stability through ubiquitination. Related references: PMID:26347139, PMID:36359729
• Plays a role in antiviral innate immunity by mediating 'Lys-63'-linked ubiquitination of NMI to facilitate NMI-IFI35 complex formation, 'Lys-6'-linked ubiquitination of IFI27 to promote pyroptosis, and 'Lys-27'-linked ubiquitination of MAVS to enhance TBK1 recruitment. Related references: PMID:26342464, PMID:36426955, PMID:29743353
• Localizes to the cytoplasm, nucleus, autophagosomes, P-bodies, and stress granules, consistent with its diverse functional roles.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 86-334, a region encompassing the central coiled-coil domain and part of the B30.2/SPRY domain of TRIM21, which is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For immunoblotting (WB), consider using appropriate protein extraction and loading controls; the predicted molecular weight is 54 kDa, but post-translational modifications may alter migration.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), validate the antibody's specificity by comparing signal in wild-type versus knockout/knockdown models if available, and optimize antigen retrieval for paraffin-embedded sections.
• For immunoprecipitation (IP) and ELISA, confirm the antibody's performance under native conditions with a relevant positive control lysate or recombinant protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio of validated antibodies, including this Anti-TRIM21/SS-A polyclonal antibody, tailored for autoimmune diagnostic research and ubiquitination pathway studies. We support diagnostic manufacturers and research labs with bulk ancillary reagents, optimized antibody pairs, and sourcing for rare-target raw materials, ensuring seamless integration from concept to clinic.