Anti-TRIF/TICAM1 Rabbit Polyclonal Antibody for WB, ELISA - Q8IUC6

Core Product Specifications and Parameters

Biological Background: TRIF/TICAM1 Function and Localization

• TRIF/TICAM1 is a TIR domain-containing adapter molecule essential for innate immunity against pathogens, acting downstream of Toll-like receptors (TLRs) and cytosolic sensors.
• Ligand engagement of TLR3, TLR4 (via TICAM2) or TLR5 triggers TRIF recruitment via its TIR domain, activating NF-kappa-B, IRF transcription factors, and apoptosis (PubMed:12471095, PubMed:12539043, PubMed:14739303). Related references: PMID:12471095, PMID:12539043, PMID:14739303
• Through distinct interaction motifs, TRIF assembles signaling complexes containing TBK1, TRAF6 and RIPK1, which respectively activate IRF3/IRF7, NF-kappa-B and FADD-mediated apoptosis (PubMed:12471095, PubMed:12539043, PubMed:14739303). Related references: PMID:12471095, PMID:12539043, PMID:14739303
• TBK1-mediated phosphorylation of the pLxIS motif in TRIF enables IRF3 binding and activation, leading to type I interferon production critical for antiviral defense (PubMed:25636800). Related references: PMID:25636800
• TRIF also participates in a multi-helicase complex that serves as a cytoplasmic sensor of viral dsRNA, triggering pro-inflammatory cytokines (By similarity).
• TRIF is found in cytoplasmic vesicles, autophagosomes, the cytosol and mitochondria, consistent with its role in multiple signaling platforms.
• Ubiquitously expressed, TRIF shows elevated levels in the liver, and is subject to post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–200 within the N-terminal region of human TRIF/TICAM1 (NP_891549.1), which is predicted to be highly antigenic and conserved across the specified species cross-reactivity (Human, Mouse, Rat).
• For Western blot (WB), detect a predominant band near 76 kDa; consider using appropriate protein loading controls and validate in lysates from TLR-stimulated cells.
• For ELISA, the rabbit polyclonal antibody can be used as a capture or detection reagent; optimal working dilutions should be empirically determined using recombinant TRIF protein or natural target from human, mouse, or rat samples.
• Due to the polyclonal nature, multiple epitopes may be recognized; include a negative control (e.g., TRIF-knockout lysate) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of IVD raw materials and research reagents, supporting diagnostic manufacturers and laboratories from early-stage development to commercialization. With expertise in innate immunity targets such as TRIF/TICAM1, we offer validated antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Our one-stop supply model ensures consistent quality and streamlined procurement, enabling accelerated advancement in immunology and infectious disease diagnostics.