Anti-TRIF / TICAM1 Rabbit Polyclonal Antibody for WB, ELISA - Q8IUC6

Core Product Specifications and Parameters

Biological Background: TRIF / TICAM1 Function and Localization

• TRIF/TICAM1 is a TIR domain-containing adapter protein pivotal in innate immune signaling, linking TLR3, TLR4 (via TICAM2), and TLR5 to the activation of NF-κB and IRF transcription factors, thereby driving pro-inflammatory and antiviral responses. Related references: PMID:12471095 PMID:12539043 PMID:14739303
• Following receptor engagement, TRIF recruits the effector proteins TBK1, TRAF6, and RIPK1, which respectively activate the IRF3/IRF7, NF-κB, and FADD-mediated apoptotic pathways, coordinating a multifaceted innate immune response. Related references: PMID:12471095 PMID:12539043 PMID:14739303
• TBK1-mediated phosphorylation of TRIF at the pLxIS motif creates a docking site for IRF3, promoting its activation and subsequent induction of type I interferons, a critical mechanism for antiviral defense. Related references: PMID:25636800
• TRIF participates in a cytoplasmic multi-helicase complex that senses viral double-stranded RNA (dsRNA), triggering a cascade of antiviral responses that includes the induction of pro-inflammatory cytokines (By similarity).
• Subcellularly, TRIF localizes to cytoplasmic vesicles, autophagosomes, the cytosol, and mitochondria, enabling spatially distinct signaling platforms.
• TRIF is ubiquitously expressed across human tissues, with relatively higher expression levels observed in the liver.
• Key functional annotations for TRIF include antiviral defense, apoptosis, host-virus interaction, inflammatory response, and innate immunity, highlighting its central role in pathogen defense.

Experimental Guidance and Technical Tips

• For Western blot (WB) analysis, a band near 76 kDa corresponding to TRIF may be detected; consider validating the signal in human, mouse, or rat tissue lysates or cell lines expressing the target.
• The immunogen spans amino acids 180–280 within the TIR domain, a region critical for receptor interaction; ensure that the antibody’s specificity is confirmed in the relevant experimental system.
• When performing ELISA, optimize the antibody dilution through pilot titration experiments to achieve the desired signal-to-noise ratio.
• As a polyclonal antibody, minor lot-to-lot variations are possible; include appropriate positive and negative controls in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. This anti-TRIF / TICAM1 rabbit polyclonal antibody supports research into innate immunity, TLR signaling, and antiviral response pathways, and CamelBio can supply the necessary complementary reagents and technical support to streamline your assay development and production workflows.