Anti-TRIB3 Rabbit Monoclonal Antibody for WB and ELISA - Q96RU7

Core Product Specifications and Parameters

Biological Background: TRIB3 Function and Localization

• TRIB3 (Tribbles homolog 3) is an inactive protein kinase that acts as a key regulator of the integrated stress response (ISR), a cellular adaptation to various stresses. It inhibits the transcriptional activity of DDIT3/CHOP and is involved in ER stress-induced cell death. Related references: PMID:15775988 PMID:15781252
• Functions as a negative feedback regulator of ATF4-dependent transcription: TRIB3 expression is induced by ATF4, and the protein then binds ATF4 to suppress its transcriptional activity, fine-tuning the ISR.
• Disrupts insulin signaling by directly binding to Akt kinases and blocking their activation, potentially by masking the Thr-308 phosphorylation site in AKT1.
• Interacts with the NF-kappa-B transactivator p65/RELA, inhibiting its phosphorylation and transcriptional activation, thereby modulating inflammatory and survival pathways. Related references: PMID:12736262
• Regulates MAP kinase activation via interaction with MAPK kinases, linking stress signals to cell proliferation and differentiation. Related references: PMID:15299019
• Can inhibit APOBEC3A editing of nuclear DNA, suggesting a role in maintaining genome stability. Related references: PMID:22977230
• Localizes predominantly to the nucleus.
• Highest expression in liver, pancreas, peripheral blood leukocytes, and bone marrow; also highly expressed in numerous primary lung, colon, and breast tumors.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal region (amino acids 1-100) of human TRIB3, making it suitable for detecting the full-length protein in western blotting and for peptide-based ELISA formats.
• A band of approximately 40 kDa is expected in western blot. Optimal antibody concentrations should be determined empirically; a starting dilution of 1:1000–1:5000 is often suitable, and validation with appropriate positive and negative controls is recommended.
• For ELISA, ensure the peptide epitope is accessible; both direct and sandwich formats may be explored depending on the assay design.
• Pre-adsorption with the immunizing peptide can serve as an effective negative control to confirm signal specificity.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support your TRIB3-related research and assay development. We specialize in sourcing rare-target raw materials and offer a seamless supply chain from concept to clinic. Contact us to discover how CamelBio can accelerate your diagnostic or laboratory projects.