Anti-TRIB2 Polyclonal Antibody for WB, ELISA - Q92519

Core Product Specifications and Parameters

Biological Background: TRIB2 Function and Localization

• TRIB2, also known as Tribbles homolog 2 (C5FW, TRB2, GS3955), is a human protein encoded by the TRIB2 gene (GeneID: 28951).
• As a pseudokinase, TRIB2 lacks detectable kinase activity but functions as an adaptor protein that interacts with MAPK kinases and modulates MAP kinase signaling cascades.
• TRIB2 acts as a protein kinase inhibitor, regulating signal transduction pathways involved in cell proliferation, differentiation, and apoptosis.
• Subcellular fractionation and immunofluorescence studies indicate TRIB2 localizes primarily to the cytoplasm and associates with the cytoskeleton.
• TRIB2 is highly expressed in peripheral blood leukocytes, suggesting a role in immune cell function.
• The immunogen used for this antibody corresponds to the N-terminal region (amino acids 1-120) of human TRIB2, which is conserved in mouse, enabling cross-reactivity.
• Structural studies have provided a 3D-structure of TRIB2, aiding understanding of its scaffolding and regulatory interactions.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant fusion protein spanning the N-terminal 120 amino acids, is designed to recognize total TRIB2. For western blot (WB), a band around 39-40 kDa is expected based on predicted molecular weight.
• This polyclonal antibody is raised in rabbit and may be suitable for detecting TRIB2 in human and mouse samples. For ELISA, consider direct coating of the immunogen or cell lysates as a screening tool.
• Validation in your specific experimental system is recommended, as cross-reactivity with other TRIB family members (TRIB1, TRIB3) or unrelated proteins may occur.
• Antibody performance may vary depending on sample preparation, blocking conditions, and detection reagents; optimize parameters for your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials and technical support, helping diagnostic developers and researchers bridge the gap from target discovery to clinical assay validation. The TRIB2 polyclonal antibody exemplifies our capability to source validated reagents for immunoassay development, including bulk quantities of antibodies, antigen pairs, and ancillary reagents. Whether you need monoclonal alternatives, optimized pairs, or rare target raw materials, our one-stop sourcing model streamlines procurement and accelerates your R&D timeline.