Anti-TRIB1 Rabbit Polyclonal Antibody for WB, ELISA - Q96RU8

Core Product Specifications and Parameters

Biological Background: TRIB1 Function and Localization

• TRIB1 is an adapter protein that interacts with the COP1 ubiquitin ligase to target the transcription factor CEBPA for degradation, playing a pivotal role in regulating myeloid cell differentiation. Related references:
  PMID:27041596

• The COP1-binding motif of TRIB1 is masked by autoinhibitory interactions with its own pseudokinase domain; binding to the CEBPA recognition sequence relieves this inhibition, modulating substrate specificity. Related references:
  PMID:26455797

• TRIB1 controls the differentiation of macrophages, eosinophils, and neutrophils through its COP1-binding domain (By similarity).

• It interacts with MAPK kinases and can regulate MAP kinase activation, yet TRIB1 itself possesses no intrinsic kinase activity. Related references:
  PMID:15299019
  PMID:26455797

• TRIB1 is expressed in a wide range of human tissues, with the highest levels observed in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow.

• The protein features a pseudokinase domain and undergoes alternative splicing; UniProt keywords include '3D-structure', 'Alternative splicing', and 'Protein kinase inhibitor'.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 203–372 of human TRIB1 (NP_079471.1), a region that includes part of the pseudokinase domain. Verify specificity by running positive and negative lysates in WB.
• For Western blot, a band at approximately 41 kDa is expected. Use reducing SDS-PAGE and appropriate controls.
• This polyclonal antibody has been validated for ELISA; determine optimal dilution for your specific assay format.
• Cross-reactivity has been confirmed for human TRIB1; reactivity with other species or isoforms has not been tested. Perform sequence alignment to predict potential cross-reactivity.
• The antibody may detect both full-length TRIB1 and alternatively spliced variants; confirm by mass spectrometry or knockdown if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials, including validated antibodies like this TRIB1 pAb. For projects exploring myeloid differentiation, leukemia, or MAPK pathway studies, we can supply optimized monoclonal/polyclonal antibodies, recombinant proteins, and ancillary reagents. Our one-stop sourcing model supports your assay development pipeline, from concept to clinic, offering bulk procurement and technical consultation.