Anti-TRIAP1 Polyclonal Antibody for WB, ELISA - O43715

Core Product Specifications and Parameters

Biological Background: TRIAP1 Function and Localization

• TRIAP1 (TP53-regulated inhibitor of apoptosis 1), also known as 15E1.1, WF-1, or p53CSV, is a small mitochondrial intermembrane space protein that plays a key role in apoptosis regulation.
• It promotes cell survival by ensuring the accumulation of cardiolipin in mitochondrial membranes; the TRIAP1:PRELID1 complex mediates phosphatidic acid (PA) transfer across the intermembrane space, supplying PA for cardiolipin synthesis in the inner membrane.
  Related references:
  PMID:23931759
  PMID:26071602
• TRIAP1 directly inhibits the activation of caspase-9, thereby preventing the induction of apoptosis downstream of mitochondrial outer membrane permeabilization.
  Related references:
  PMID:15735003
• The protein localizes exclusively to the mitochondrion intermembrane space, consistent with its role in lipid shuttling and apoptotic control.
• The TRIAP1:PRELID3A complex also mediates PA transfer in vitro and is likely a PA transporter in vivo, linking TRIAP1 to broader mitochondrial lipid metabolism.
• Known post-translational modifications include acetylation, and the protein contains a coiled-coil domain and a disulfide bond, which may be important for its structural stability and interactions.
• Keywords associated with TRIAP1 include apoptosis, lipid transport, and mitochondrion, underscoring its central role in mitochondrial-mediated cell death and lipid homeostasis.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein encompassing the entire TRIAP1 sequence (aa 1-76), which may offer broad epitope coverage; however, consider that polyclonal antibodies can exhibit lot-to-lot variation.
• For WB, the predicted molecular weight of TRIAP1 is ~9 kDa; careful optimization of gel percentage and transfer conditions is recommended for small proteins.
• This antibody has been reported for use in WB and ELISA against human and mouse samples; cross-reactivity with other species is not specified and should be verified experimentally.
• As TRIAP1 is a mitochondrial protein, sample preparation should include mitochondria-enriched fractions to enhance detection sensitivity if expression levels are low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and laboratories a streamlined procurement pathway for high-quality IVD raw materials. This TRIAP1 rabbit polyclonal antibody is suitable for apoptosis research and assay development, and complements our extensive portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents. Leverage our expertise to efficiently source rare-target antibodies and build robust diagnostic workflows from concept to clinic.