Anti-TRHR Rabbit Polyclonal Antibody for WB, ELISA - P34981

Core Product Specifications and Parameters

Biological Background: TRHR Function and Localization

• TRHR (Thyrotropin-releasing hormone receptor), also known as thyroliberin receptor, is a G protein-coupled receptor encoded by the TRHR gene in humans.
• It serves as the receptor for thyrotropin-releasing hormone (TRH). Upon ligand binding, TRHR activates the phosphatidylinositol (IP3)-calcium-protein kinase C (PKC) signaling pathway, leading to downstream cellular responses.
• As a transmembrane protein, TRHR is localized at the cell membrane.
• TRHR belongs to the G protein-coupled receptor superfamily and contains structural features such as disulfide bonds and glycosylation sites, which are important for its stability and function.
• Genetic variations in TRHR are linked to congenital hypothyroidism, underscoring its critical role in thyroid function and development.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein corresponding to the C-terminal region (amino acids 320–398) of human TRHR. This design may aid in detecting total TRHR protein in applications such as Western blot and ELISA.
• For Western blot, the predicted molecular weight of TRHR is approximately 45 kDa. Consider validating band specificity using positive and negative control lysates.
• As a rabbit polyclonal antibody, it may recognize multiple epitopes; verify lot-to-lot consistency for quantitative assays.
• Cross-reactivity is reported for human TRHR. For other species, assess sequence homology of the immunogen region before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, including validated antibodies and ancillary reagents. This anti-TRHR antibody, targeting a key GPCR involved in thyroid hormone signaling, supports research into endocrine disorders and related assay development. As your sourcing partner, CamelBio helps streamline procurement from discovery to commercial-scale supply.