Anti-TREX1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NSU2

Core Product Specifications and Parameters

Biological Background: TREX1 Function and Localization

• TREX1 is a major cellular 3'-to-5' DNA exonuclease that digests single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) with mismatched 3' termini, preventing cell-intrinsic initiation of autoimmunity by metabolizing DNA fragments from endogenous retroelements including L1, LTR and SINE elements. Related references: PMID:10391904 PMID:10393201 PMID:17293595
• Upon genome instability, TREX1 is recruited from the endoplasmic reticulum membrane to ruptured micronuclei, where it degrades micronuclear DNA to limit cGAS activation and subsequent type I interferon production, thereby preventing autoinflammatory responses. Related references: PMID:33476576
• TREX1 processes ssDNA polynucleotide species arising from the processing of aberrant DNA replication intermediates, preventing chronic ATM-dependent checkpoint activation. Related references: PMID:18045533
• Inefficient degradation of oxidized DNA, such as that generated upon antimicrobial reactive oxygen production or UV absorption, may contribute to immune activation under oxidative stress conditions. Related references: PMID:23993650
• During GZMA-mediated cell death, TREX1 cooperates with NME1 to enhance DNA damage: NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end, preventing DNA end reannealing and rapid repair. Related references: PMID:16818237
• TREX1 localizes to the nucleus, cytoplasm, cytosol, and endoplasmic reticulum membrane.
• Detected in thymus, spleen, liver, brain, heart, small intestine and colon.
• Mutations in TREX1 are associated with Aicardi-Goutieres syndrome, systemic lupus erythematosus, and other neurodegenerative disorders.

Experimental Guidance and Technical Tips

• For Western blot (WB), use whole cell lysates from human, mouse, or rat tissues known to express TREX1. The predicted molecular weight is ~33 kDa; validate with positive and negative controls.
• For immunofluorescence (IF/ICC), optimize fixation and permeabilization protocols to detect TREX1 in its nuclear and cytoplasmic compartments. Consider co-localization with organelle markers.
• For ELISA, this polyclonal antibody may be used as a capture or detection reagent; test pairing with a monoclonal antibody specific to a distinct epitope. Validate assay performance with recombinant TREX1 protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated partner for IVD raw materials, providing reliable access to high-quality antibodies and research reagents for DNA damage response and autoimmune disease studies. Our portfolio includes this polyclonal TREX1 antibody, as well as monoclonal antibody pairs, optimized primary and secondary antibodies, bulk ancillary reagents, and sourcing for rare target raw materials. From early-stage development to clinical application, CamelBio bridges the gap between concept and clinic with a streamlined, one-stop solution.