Anti-TREM2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NZC2

Core Product Specifications and Parameters

Biological Background: TREM2 Function and Localization

• TREM2 is a single-pass type I membrane protein that contains an extracellular immunoglobulin-like domain and forms a signaling complex with TYROBP to mediate cell activation upon ligand binding. Related references: PMID:10799849
• It acts as a receptor for amyloid-beta 42, promoting its uptake and degradation in microglia, a process critical for amyloid clearance in the brain. Related references: PMID:27477018, PMID:29518356
• TREM2 also binds lipoprotein particles (LDL, VLDL, HDL) and apolipoproteins (e.g., APOE, CLU), facilitating lipid uptake and contributing to microglial lipid metabolism. Related references: PMID:27477018
• It regulates microglial functions, including phagocytosis of apoptotic neurons, chemotaxis, process outgrowth, and response to oxidative stress. Related references: PMID:24990881
• The protein is predominantly localized on the cell membrane but can also be secreted; it undergoes glycosylation and contains disulfide bonds that stabilize its structure.
• Highly expressed in brain microglia, macrophages, and dendritic cells, with strong presence in the basal ganglia, corpus callosum, medulla oblongata, and spinal cord.
• Mutations in TREM2 are linked to neurodegenerative disorders such as Alzheimer's disease and frontotemporal dementia, underscoring its role in neuroinflammation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 19–161), which is expected to generate antibodies recognizing both membrane-bound and secreted TREM2; consider this when designing detection strategies.
• For Western blot (WB), a band at ~25 kDa is anticipated; use appropriate positive controls (e.g., human microglial lysates) and loading controls for reliable quantification.
• In immunofluorescence/ICC, the antibody may be suitable for surface staining or intracellular detection depending on fixation/permeabilization; validate in your specific cellular model.
• For ELISA, the polyclonal nature may enhance sensitivity; however, carefully titrate to minimize background and confirm specificity.
• Reported cross-reactivity with mouse and rat TREM2 makes this antibody useful for translational studies; still, verify reactivity in your tissue/cell line due to potential sequence variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting. For neuroscience and Alzheimer's research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, ensuring smooth progression from discovery to clinical application. Whether you need TREM2 antibodies or rare-target sourcing, CamelBio is your reliable procurement partner.