Anti-TREM1 Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q9NP99

Core Product Specifications and Parameters

Biological Background: TREM1 Function and Localization

• TREM1 is a cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses. Related references: PMID:10799849, PMID:21393102
• Upon activation by ligands such as PGLYRP1, HMGB1, or HSP70, TREM1 multimerizes and forms a signaling complex with the transmembrane adaptor TYROBP/DAP12. Related references: PMID:17568691, PMID:25595774, PMID:29568119
• This complex initiates a SYK-mediated tyrosine phosphorylation cascade, activating downstream mediators including BTK, MAPK1, MAPK3, and phospholipase C-gamma. Related references: PMID:14656437, PMID:21659545
• The signaling cascade promotes the release of pro-inflammatory cytokines and chemokines, as well as neutrophil and macrophage migration, thereby amplifying responses to bacterial and fungal infections. Related references: PMID:17098818, PMID:17568691
• TREM1 also amplifies inflammatory signals initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, playing a major role in septic shock and atherosclerosis. Related references: PMID:11323674, PMID:21393102
• A secreted isoform of TREM1 acts as a decoy receptor, neutralizing its ligands and counterbalancing pro-inflammatory activity.
• TREM1 localizes to the cell membrane and is also shed as a soluble form; it contains an immunoglobulin-like domain and is glycosylated.
• TREM1 is predominantly expressed on myeloid lineage cells—monocytes, macrophages, neutrophils, and dendritic cells—and is associated with mature myeloid development. Isoform 2 is detected in lung, liver, and mature monocytes.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a synthetic peptide corresponding to amino acids 1-100 of human TREM1; it may recognize both full-length membrane-bound and secreted/soluble isoforms.
• For immunofluorescence/ICC applications, consider optimizing fixation and permeabilization protocols to detect intracellular or membrane-localized TREM1. Include appropriate controls to confirm staining specificity.
• In ELISA, the antibody may be used for capture or detection; validate with recombinant human TREM1 protein and test in your sample matrix to establish assay sensitivity and dynamic range.
• Due to the polyclonal nature, cross-reactivity with closely related TREM family members is possible; check sequence alignment of the immunogen against other human TREMs and validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive, one-stop sourcing solutions for IVD raw materials, supporting diagnostic manufacturers and research labs from concept to clinic. This anti-TREM1 polyclonal antibody suits innate immunity and inflammatory disease research, and CamelBio can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our platform also assists with rare target raw-material sourcing, ensuring you have reliable access to critical immunoreagents.