Anti-TREM1 Monoclonal Antibody for FC - Q9NP99

Core Product Specifications and Parameters

Biological Background: TREM1 Function and Localization

• TREM1 is a cell surface receptor that amplifies inflammatory responses, playing key roles in both innate and adaptive immunity. Related references: PMID:10799849 PMID:21393102
• Upon ligand binding (e.g., PGLYRP1, HMGB1, HSP70), it oligomerizes and recruits TYROBP/DAP12 to initiate SYK-mediated phosphorylation cascades, activating downstream mediators like BTK, MAPK1/3, and PLC-gamma. Related references: PMID:17568691 PMID:25595774 PMID:29568119 PMID:14656437 PMID:21659545
• This signaling drives the release of pro-inflammatory cytokines and chemokines from neutrophils and macrophages and promotes their migration, especially in response to bacterial and fungal infections. Related references: PMID:17098818 PMID:17568691
• TREM1 enhances inflammatory signals from Toll-like receptors (TLRs) and NOD-like receptors, contributing to the pathology of acute and chronic inflammatory diseases, including septic shock and atherosclerosis. Related references: PMID:11323674 PMID:21393102
• An alternative splice isoform (isoform 2) is secreted and functions as a decoy receptor, neutralizing TREM1 ligands to dampen pro-inflammatory activity.
• TREM1 is predominantly expressed on myeloid lineage immune cells (monocytes, macrophages, neutrophils, dendritic cells) and is associated with mature developmental stages. Its expression is higher in adult liver, lung, spleen compared to fetal tissue, and it is also found in lymph node, placenta, spinal cord, and heart. Related references: PMID:10799849 PMID:11922939
• The protein is modified by glycosylation and disulfide bond formation, features typical of immunoglobulin domain-containing receptors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21-200) of human TREM1, which is suitable for generating antibodies recognizing native surface receptor.
• For flow cytometry, consider using this antibody to detect TREM1 on live, unfixed myeloid cells; validate the optimal concentration in your experimental system.
• The antibody may also be tested in other applications that detect native protein, such as functional neutralization assays, though this requires independent validation.
• Since TREM1 has a secreted isoform, be aware that the antibody may recognize both membrane-bound and soluble forms; use appropriate controls to distinguish them.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw-material sourcing for diagnostic manufacturers and research labs, from discovery to commercialization. This anti-TREM1 monoclonal antibody exemplifies our portfolio for innate immunity and inflammation studies. Beyond single antibodies, we provide matched pairs, bulk aliquots, and access to difficult-to-source targets to accelerate your assay development.