Anti-TRAPPC6A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O75865

Core Product Specifications and Parameters

Biological Background: TRAPPC6A Function and Localization

• TRAPPC6A (Trafficking protein particle complex subunit 6A), also known as TRS33, is a component of the TRAPP (transport protein particle) complex involved in vesicular trafficking.
• The protein may play a role in the biogenesis of melanosomes, which are lysosome-related organelles responsible for melanin synthesis and storage.
• Alternative splicing generates multiple transcript variants, contributing to potential functional diversity.
• TRAPPC6A localizes to the Golgi apparatus, specifically the cis-Golgi network, and the endoplasmic reticulum, consistent with its role in early secretory pathway trafficking.
• Post‑translational modification includes phosphorylation, as indicated by proteomics data and keyword annotation.
• The protein is implicated in ER‑to‑Golgi transport, a critical step in protein secretion and organelle biogenesis.
• Structural studies have elucidated its 3D conformation, offering insights into its interaction within the TRAPP complex.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full‑length human TRAPPC6A (residues 1‑173); the antibody is expected to recognize all isoforms containing this region.
• For Western blotting, a band around 18 kDa is anticipated in lysates from human, mouse, or rat samples. Optimize loading and transfer conditions for small proteins.
• In immunofluorescence/ICC, fixation and permeabilization protocols should be optimized to detect Golgi and ER localization; co‑staining with organelle markers is recommended.
• ELISA applications may leverage the polyclonal nature for enhanced sensitivity in antigen capture or detection formats; validate with recombinant protein or native samples.
• Cross‑reactivity with mouse and rat suggests conservation of the epitope region; verify in the relevant sample system if working with other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and researchers with streamlined access to IVD raw materials, including this anti‑TRAPPC6A antibody for studies on vesicular transport and melanosome biology. We offer validated polyclonal antibodies, bulk ancillary reagents, and sourcing for rare‑target raw materials to accelerate your assay development. Partner with CamelBio to bridge the gap from concept to clinic with reliable, one‑stop procurement solutions.