Anti-TRAPPC10 Polyclonal Antibody for WB, ELISA - P48553

Core Product Specifications and Parameters

Biological Background: TRAPPC10 Function and Localization

• TRAPPC10 (Trafficking protein particle complex subunit 10) is also known as EHOC-1, GT334, TMEM1, and TRS130; it is the subunit of the highly conserved TRAPP II complex.
• As a specific component of the TRAPP II complex, TRAPPC10 functions in late Golgi trafficking as a membrane tether, mediating vesicle tethering during ER-to-Golgi and intra-Golgi transport.
• TRAPPC10 belongs to the TRAPP complex family, which includes multiple subunits that assemble into distinct complexes (TRAPP I, II, III) to regulate different trafficking steps.
• The protein localizes primarily to the Golgi apparatus, specifically the cis-Golgi network, consistent with its role in early secretory pathway vesicle tethering.
• TRAPPC10 is expressed in all tissues examined, indicating a ubiquitous role in intracellular trafficking.
• Alternative splicing generates multiple isoforms, and post-translational modifications include phosphorylation (Phosphoprotein keyword).
• Mutations in TRAPPC10 are associated with neurodevelopmental disorders, including epilepsy, autism spectrum disorder, intellectual disability, and dwarfism, highlighting its importance in neuronal function.
• Keywords from UniProt also include disease variant, proteomics identification, and reference proteome, reflecting its clinical relevance and extensive characterization.

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region (amino acids 1-260) of human TRAPPC10; for Western blot, a band at approximately 142 kDa is expected—validate in appropriate human, mouse, or rat cell lysates or tissue extracts.
• Due to the antibody's polyclonal nature, multiple bands may be observed; consider using blocking buffers and optimizing antibody dilution for cleaner results.
• When performing ELISA, ensure proper coating of the recombinant protein or target antigen, and include relevant controls to confirm specificity.
• Cross-reactivity with mouse and rat suggests this antibody can be used for rodent model studies; however, always verify performance in your specific experimental system.
• For immunohistochemistry or other applications not listed, pilot testing is recommended, as this antibody has been validated only for WB and ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-TRAPPC10 polyclonal antibody supports research into Golgi trafficking and neurodevelopmental disorders, and CamelBio can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your assay development. We serve as your procurement bridge, ensuring reliable access to high-quality reagents for your diagnostic and research needs.