Anti-TRAP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC - Q12931

Core Product Specifications and Parameters

Biological Background: TRAP1 Function and Localization

• TRAP1, also known as Heat shock protein 75 kDa, mitochondrial (HSP75), HSPC5, and TNFR-associated protein 1, is a member of the HSP90 family of molecular chaperones.
• It possesses ATPase activity and functions as a chaperone involved in maintaining mitochondrial integrity and polarization, acting downstream of PINK1 and mitochondrial complex I.
• TRAP1 acts as a negative regulator of mitochondrial respiration, modulating the balance between oxidative phosphorylation and aerobic glycolysis, likely through modulation of mitochondrial SRC and inhibition of SDHA.
• The protein is localized primarily to the mitochondrion, with sub-mitochondrial distribution in the inner membrane and matrix.
• TRAP1 is broadly expressed in tissues such as skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta, and bladder; its expression is reduced in bladder and renal cell carcinomas but elevated in other tumor types.
• Key post-translational modifications include phosphorylation and acetylation, and the protein undergoes alternative splicing; it is synthesized with a transit peptide for mitochondrial import.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 445–704 of human TRAP1 (C-terminal region), which may contribute to specific recognition. For Western blotting, an expected band around 80 kDa should be observed; minor additional bands might appear due to post-translational modifications or isoforms.
• For IHC-P and IF/ICC, appropriate antigen retrieval and fixation methods should be optimized for the tissue or cell type. The antibody’s cross-reactivity with mouse and rat suggests utility across common model organisms, but validation in the specific experimental context is recommended.
• When setting up ELISA, consider the polyclonal nature—possible lot-to-lot variation may require titration and careful interpretation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated B2B partner providing diagnostic manufacturers, laboratories, and research institutions with a comprehensive range of IVD raw materials. This anti-TRAP1 antibody exemplifies our commitment to supplying quality polyclonal antibodies for mitochondrial biology and oncology research. Beyond individual reagents, we offer bulk ancillary reagents, validated antibody pairs, and customized monoclonal/polyclonal antibody sourcing to streamline your diagnostic development pipeline from concept to clinic.