Anti-TRAP1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IF-P, IHC-P, ELISA - Q12931

Core Product Specifications and Parameters

Biological Background: Heat shock protein 75 kDa, mitochondrial Function and Localization

• Heat shock protein 75 kDa, mitochondrial (TRAP1/HSP75) is a member of the heat shock protein 90 family functioning as an ATP-dependent molecular chaperone.
• It plays a critical role in maintaining mitochondrial integrity and polarization, acting downstream of PINK1 and mitochondrial complex I.
• TRAP1 negatively regulates mitochondrial respiration, modulating the balance between oxidative phosphorylation and aerobic glycolysis, possibly through inhibition of succinate dehydrogenase (SDHA) and modulation of mitochondrial SRC.
• The protein localizes predominantly to the mitochondrial inner membrane and matrix, consistent with its role in mitochondrial homeostasis.
• Tissue expression is broad, including skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta, and bladder; expression levels are reduced in bladder cancer and renal cell carcinoma but elevated in several other tumor types.
• Post-translational modifications include acetylation and phosphorylation, and the protein features a transit peptide for mitochondrial import.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 445-704) of human TRAP1; consider using this information to confirm detection of full-length protein or specific isoforms.
• For Western blotting, an expected band around 80 kDa should be observed; validate in relevant mitochondrial-enriched samples.
• This monoclonal antibody can be applied in immunoprecipitation, immunofluorescence, and immunohistochemistry; optimize fixation and permeabilization conditions for mitochondrial targets.
• Cross-reactivity with mouse is reported; verify performance in mouse-derived samples if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized one-stop supplier supporting diagnostic manufacturers and research laboratories with validated IVD raw materials. This anti-TRAP1 monoclonal antibody exemplifies our offering in the areas of mitochondrial biology and cancer metabolism research. In addition to this product, we can source validated antibody pairs, optimized monoclonal and polyclonal antibodies in bulk, ancillary reagents, and rare-target raw materials to meet your development and scaling needs.