Anti-TRAP1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q12931

Core Product Specifications and Parameters

Biological Background: TRAP1 Function and Localization

• TRAP1, also known as Heat shock protein 75 kDa, mitochondrial, Hsp75, HSP90L, or TNFR-associated protein 1, is a mitochondrial chaperone with ATPase activity.
• It maintains mitochondrial function and polarization downstream of PINK1 and mitochondrial complex I, acting as a negative regulator of mitochondrial respiration to modulate the balance between oxidative phosphorylation and aerobic glycolysis.
• TRAP1 belongs to the heat shock protein 90 family and is encoded by the TRAP1 gene (also known as HSP75, HSPC5) on chromosome 16.
• It localizes to the mitochondrion, predominantly in the inner membrane and matrix.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.
• TRAP1 expression is found in various tissues including skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta, and bladder, with altered expression in certain cancers such as reduced levels in bladder and renal cell carcinoma but increased in other tumors.
• The protein has a molecular weight of approximately 80 kDa (calculated 80,110 Da) and may exist in multiple isoforms due to alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, consider using this antibody to detect the ~80 kDa TRAP1 band. Include appropriate controls to confirm specificity.
• When performing IHC-P or IF/ICC, validate antigen retrieval methods and fixation conditions for your specific sample type to ensure optimal signal-to-noise ratio.
• The immunogen covers amino acids 445-704, so the antibody may recognize multiple isoforms or protein fragments containing this region; additional validation may be required for isoform-specific detection.
• For ELISA applications, use the recommended coating buffer and consider cross-reactivity testing with related hsp90 family members.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers, laboratories, and research institutes streamlined access to validated antibodies and ancillary reagents. This TRAP1 antibody supports research in mitochondrial biology, chaperone function, and cancer. Our supply capabilities extend to optimized monoclonal/polyclonal antibodies, bulk reagents, and custom sourcing for rare targets, enabling efficient development from concept to clinic.