Anti-Transthyretin (TTR) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P02766

Core Product Specifications and Parameters

Biological Background: Transthyretin Function and Localization

• Transthyretin (TTR), also known as prealbumin, ATTR, or TBPA, is encoded by the TTR gene (formerly PALB) in humans.
• It functions as a thyroid hormone-binding protein, primarily transporting thyroxine from the bloodstream to the brain.
• The protein is detected in serum and cerebrospinal fluid, with high expression in choroid plexus epithelial cells; it is also present in retinal pigment epithelium and liver.
• Subcellularly, TTR is secreted and can also be found in the cytoplasm.
• Keywords associated with TTR include amyloid and amyloidosis, as mutations in the TTR gene are linked to familial amyloidotic polyneuropathy and other amyloid-related diseases.
• Post-translational modifications include phosphorylation, sulfation, and gamma-carboxyglutamic acid formation.
• The mature protein has a molecular weight of approximately 15.9 kDa (UniProt), consistent with the ~16 kDa band observed in Western blot.

Experimental Guidance and Technical Tips

• The immunogen (aa 21-147) corresponds to the mature secreted form of TTR; this region is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, a single band of approximately 16 kDa is expected in reduced samples. Serum or liver lysates from human, mouse, or rat can serve as positive controls.
• In IHC-P, consider using formalin-fixed paraffin-embedded liver or choroid plexus tissues; antigen retrieval may enhance detection.
• The antibody has been reported by the manufacturer in ELISA; it may be suitable for sandwich immunoassays with a paired antibody targeting a different epitope.
• Due to the presence of TTR in serum, high backgrounds in serological samples can be mitigated by appropriate blocking and dilution optimization.
• Fluorescence-based applications (IF/ICC, IF-P) require validation in the specific cell or tissue system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This TTR polyclonal antibody supports research in thyroid hormone transport, neurodegenerative diseases, and amyloidosis. Explore our portfolio for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing.