Anti-Transportin 3 (TNPO3) Rabbit Monoclonal Antibody for WB, IF/ICC, IF-P, IHC-P, ELISA - Q9Y5L0

Core Product Specifications and Parameters

Biological Background: Transportin-3 Function and Localization

• Transportin-3 (TNPO3), also known as Importin-12 and Transportin-SR, is a nuclear transport receptor that specifically mediates the nuclear import of serine/arginine-rich (SR) proteins by recognizing phosphorylated SR domains on cargoes such as RBM4, SFRS1, and SFRS2. Related references: PMID:10366588, PMID:10713112, PMID:11517331
• TNPO3 also mediates the nuclear import of CPSF6 in a phosphorylation-independent manner, demonstrating broader substrate specificity. Related references: PMID:30916345, PMID:31465518
• The nuclear import cycle is regulated by the small GTPase Ran; TNPO3 binds cargo in the cytoplasm and releases it upon RanGTP binding in the nucleus. Related references: PMID:23878195, PMID:24449914
• TNPO3 is hijacked by HIV-1 to facilitate nuclear import of the viral pre-integration complex and is required for a nuclear maturation step prior to integration. Related references: PMID:18722123, PMID:21901095, PMID:22398280
• Subcellularly, TNPO3 localizes to the nucleus envelope and cytoplasm, consistent with its role in nucleocytoplasmic shuttling.
• TNPO3 undergoes post-translational modifications including acetylation and phosphorylation, and alternative splicing produces multiple isoforms; disease-causing mutations are linked to limb-girdle muscular dystrophy type 1F.
• The protein is expressed in skeletal muscle and has a molecular weight of approximately 104 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 104 kDa is expected; use appropriate positive control lysates from human, mouse, or rat tissues expressing TNPO3, such as skeletal muscle.
• In immunofluorescence (IF/ICC and IF-P), TNPO3 typically localizes to the nuclear envelope and cytoplasm; validate staining patterns in relevant cell lines or tissue sections.
• IHC-P applications may require antigen retrieval optimization; consider testing in skeletal muscle or disease-relevant tissues given TNPO3’s association with limb-girdle muscular dystrophy.
• The antibody recognizes an epitope within amino acids 824–923 of human TNPO3; confirm compatibility with target isoforms or post-translational modifications in the experimental system.
• For ELISA, pairing with a different clone could enable sandwich assay development; validate specificity and sensitivity using recombinant TNPO3 or lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier B2B provider of IVD raw materials, offering diagnostic developers and researchers streamlined access to high-quality antibodies, antigen pairs, and ancillary reagents. For those investigating nucleocytoplasmic transport, splicing regulation, or viral pathogenesis, CamelBio supplies validated monoclonal antibodies like anti-TNPO3, along with customized sourcing for rare targets, ensuring continuity from discovery to commercial IVD assay development.