Anti-Translin (TSN) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15631

Core Product Specifications and Parameters

Biological Background: Translin Function and Localization

• Translin, also known as Component 3 of promoter of RISC (C3PO), is a DNA/RNA-binding protein encoded by the TSN gene.
• As a DNA-binding protein, it specifically recognizes consensus sequences at breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig) and T-cell receptor gene segments, and binds single-stranded DNA ends generated during recombination.
• Exhibits both single-stranded and double-stranded endoribonuclease activity, and is implicated in RNA-induced silencing complex (RISC) activation by facilitating endonucleolytic cleavage of the siRNA passenger strand.
• Localizes to both the cytoplasm and nucleus, consistent with its roles in DNA repair/recombination and RNA processing.
• Protein weight is approximately 26 kDa; the antibody detects a band around 26 kDa in WB.
• Post-translational modifications include acetylation and phosphorylation; alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 50–150 of human Translin (NP_004613.1), a region highly conserved across human, mouse, and rat, supporting the observed cross-reactivity. For species not listed, verify sequence homology before use.
• For Western blot (WB), expect a specific band at ~26 kDa. Optimize blocking and antibody dilution for your sample type.
• For IHC-P and IF/ICC, consider the cytoplasmic and nuclear localization; appropriate fixation and permeabilization protocols are recommended.
• This polyclonal antibody is suitable for ELISA; titrate the antibody to achieve an optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to support your diagnostic and research workflows. The anti-Translin polyclonal antibody targets a conserved DNA/RNA-binding protein involved in chromosomal translocation and RISC biology, making it suitable for studies in oncology, immunology, and gene regulation. Leverage CamelBio's comprehensive supply chain and technical consulting to accelerate your project from concept to clinic.