Anti-Transketolase (TKT) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P29401

Core Product Specifications and Parameters

Biological Background: Transketolase Function and Localization

• Transketolase (TKT) is a thiamine pyrophosphate-dependent enzyme that catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, forming a covalent intermediate with the cofactor. Related references: PMID:20667822, PMID:27259054, PMID:31534226
• It reversibly converts sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate to xylulose-5-phosphate and ribose-5-phosphate, a key reaction in the pentose phosphate pathway.
• The enzyme can also utilize fructose-6-phosphate as a ketose donor.
• TKT is a metal-binding protein, interacting with calcium and magnesium ions, and undergoes post-translational modifications including acetylation and phosphorylation.
• Alternative splicing of the TKT gene produces multiple isoforms.
• Mutations in TKT are associated with disease variants, including a form of dwarfism.
• The calculated molecular weight of human TKT is approximately 68 kDa.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein corresponding to the N-terminal region (aa 1-280) of human TKT, which may help generate antibodies recognizing conserved functional domains.
• For Western blotting, the predicted band size is around 68 kDa; verify expression in tissues or cell lines known to express TKT.
• When performing immunohistochemistry (IHC-P), consider antigen retrieval methods and primary antibody dilution optimization for your sample type.
• ELISA assays may be performed by coating the recombinant immunogen or native protein; titration of both capture and detection antibodies is recommended.
• Cross-reactivity with mouse and rat TKT has been indicated by the supplier; validation for your specific application is advised.

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CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, technical services, and consulting. For researchers studying metabolic pathways and enzyme kinetics, we supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and can source rare targets. Let CamelBio be your bridge from concept to clinic, supporting every stage of assay development.