Anti-Transgelin/TAGLN Rabbit PolymAb® for WB, IF-P, IHC-P, ELISA - Q01995

Core Product Specifications and Parameters

Biological Background: Transgelin/TAGLN Function and Localization

• Transgelin, also known as SM22-alpha, WS3-10, or 22 kDa actin-binding protein, is encoded by the TAGLN gene and is a member of the calponin family of actin-binding proteins.
• It functions as an actin cross-linking and gelling protein, thereby regulating actin filament dynamics and cytoskeletal organization.
• Transgelin is involved in calcium-dependent interactions and contributes to the contractile properties of the cell, which may play a role in replicative senescence.
• The protein predominantly localizes to the cytoplasm.
• It undergoes multiple post-translational modifications, including acetylation, methylation, and phosphorylation.
• Transgelin is a marker of smooth muscle differentiation and is also expressed in other cell types where it participates in actin remodeling.

Experimental Guidance and Technical Tips

• The immunogen was designed from a synthetic peptide spanning amino acids 100-201 of human TAGLN (NP_003177.2). This region shows high conservation among human, mouse, and rat, consistent with the observed cross-reactivity.
• For western blot (WB), a specific band around 23 kDa is expected. Endogenous transgelin expression levels may vary across cell and tissue types.
• In immunofluorescence (IF-P) and immunohistochemistry (IHC-P), consider optimizing permeabilization and antigen retrieval conditions for the target sample.
• ELISA can be employed for quantitative detection; appropriate blocking and washing steps should be validated.
• As a recombinant rabbit monoclonal antibody (Rabbit PolymAb®), high lot-to-lot consistency is expected, but experimental conditions should be titrated individually.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive suite of IVD raw materials and technical services. This anti-Transgelin/TAGLN antibody is a valuable tool for studies on smooth muscle biology, cytoskeletal dynamics, and replicative senescence. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing solutions to accelerate your research and development from concept to clinic.