Anti-Transgelin (TAGLN) Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - Q01995

Core Product Specifications and Parameters

Biological Background: Transgelin Function and Localization

• Transgelin (TAGLN), also known as SM22-alpha or WS3-10, is a 22 kDa actin-binding protein and a member of the calponin family.
• It functions as an actin cross-linking/gelling protein, playing a critical role in the organization of the actin cytoskeleton.
• TAGLN is involved in calcium-dependent contractile properties of smooth muscle cells, contributing to cellular contractility and replicative senescence.
• The protein is predominantly localized in the cytoplasm, consistent with its role in cytoskeletal dynamics.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its activity and interactions.
• As a muscle protein, Transgelin is highly expressed in smooth muscle tissues and serves as an early marker of smooth muscle differentiation.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering the full-length human Transgelin (amino acids 1-201), suggesting the antibody targets the whole protein. For optimal results, consider titration in your specific assay system.
• Validated applications include immunofluorescence on paraffin-embedded sections (IF-P), immunohistochemistry (IHC-P), and ELISA. Ensure proper sample preparation, such as antigen retrieval for IHC-P, and validate in the relevant sample system (e.g., smooth muscle tissues).
• This antibody cross-reacts with human, mouse, and rat, enabling multi-species studies; however, verify cross-reactivity in your experimental model, as epitope conservation may vary.
• For Western blotting, note that the predicted molecular weight is approximately 23 kDa; the antibody may also detect this band, though the application has not been validated by the supplier—consider testing under reducing conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw-material sourcing, connecting diagnostic manufacturers, labs, and research institutes with validated antibody pairs, monoclonal reagents, and bulk ancillary materials. This Transgelin antibody is available for research and assay development in the cytoskeleton and muscle biology fields. For custom or scaled-up requirements, CamelBio facilitates access to specialized raw materials and technical support, streamlining your procurement from concept to clinical application.