Anti-Transferrin Polyclonal Antibody for WB, IF, ELISA - P02787

Core Product Specifications and Parameters

Biological Background: Transferrin Function and Localization

• Transferrin, also known as Serotransferrin, Beta-1 metal-binding globulin, or Siderophilin, is a 77 kDa glycoprotein encoded by the TF gene in humans.
• It serves as the primary iron transport protein, capable of binding two Fe³⁺ ions in association with an anion, typically bicarbonate, facilitating iron delivery from absorption and heme degradation sites to storage and utilization sites.
• Serum transferrin may also stimulate cell proliferation.
• During microbial infections, transferrin acts as an iron source for pathogens such as Neisseria species and Trypanosoma brucei, which capture the protein via specific receptors to extract iron.
• Transferrin is secreted and predominantly expressed by the liver, circulating in plasma.
• Post-translational modifications include glycosylation, phosphorylation, methylation, and disulfide bond formation, which are critical for its structure and function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a large C-terminal region (amino acids 355-698), so this polyclonal antibody may recognize multiple epitopes, providing robust detection across applications.
• For Western blot, the predicted molecular weight is 77 kDa; however, due to glycosylation, the observed band may migrate higher (e.g., 80–90 kDa). Validate in the relevant sample system.
• Consider using liver-derived cell lines (e.g., HepG2) or liver tissue sections as positive controls for immunofluorescence applications.
• Antibody specificity can be confirmed by blocking with the recombinant immunogen or using transferrin-depleted samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For transferrin-related studies, we can supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and assist in sourcing rare-target raw materials to accelerate your assay development.