Anti-Transcription Factor AP-2-alpha (TFAP2A) Monoclonal Antibody for WB, ELISA - P05549

Core Product Specifications and Parameters

Biological Background: Transcription Factor AP-2-alpha Function and Localization

• Transcription factor AP-2-alpha (TFAP2A) is a sequence-specific DNA-binding protein that regulates transcription by binding to the consensus motif 5'-GCCNNNGGC-3' in promoters of target genes.
• AP-2-alpha activates genes essential for proper eye, face, body wall, limb, and neural tube development, and is the only AP-2 protein required for early lens vesicle morphogenesis.
• It also functions as a transcriptional repressor, suppressing genes such as MCAM/MUC18 and C/EBP alpha, and negatively regulates Wnt canonical signaling; AP-2-alpha is a transcriptional repressor of MYC. Related references: PMID:33000225
• The protein is localized to the nucleus where it associates with chromatin; together with coactivator CITED2, it stimulates PITX2 P1 promoter transcription activation.
• Post-translational modifications include phosphorylation and isopeptide bond formation (Ubl conjugation), suggesting complex regulatory mechanisms.
• Alternative splicing generates multiple isoforms of AP-2-alpha, expanding its functional diversity.
• Mutations in TFAP2A are associated with branchiooculofacial syndrome (BOFS), highlighting its role in developmental disorders.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic peptide spanning amino acids 50–150 of human AP-2-alpha; this region may be conserved in mouse, consistent with the reported cross-reactivity.
• For Western blot (WB), expect a band near 48 kDa; consider using nuclear-enriched lysates to improve detection of this transcription factor.
• In ELISA, the peptide immunogen can serve as a positive control, but endpoint validation with recombinant protein or cell lysates is recommended.
• Since AP-2-alpha is involved in early development and oncogenesis, including appropriate positive (e.g., HeLa, MCF-7) and negative controls in your assays is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and researchers, offering one-stop access to IVD raw materials. This anti-AP-2-alpha antibody supports studies in transcription regulation and developmental biology; beyond antibodies, we can source matched antibody pairs, bulk ancillary reagents, and assist with rare target raw-material procurement to streamline your assay development from concept to clinic.