Anti-Transcobalamin-1 (TCN1) Polyclonal Antibody for WB, ELISA - P20061

Core Product Specifications and Parameters

Biological Background: Transcobalamin-1 Function and Localization

• Encoded by the TCN1 gene, Transcobalamin-1 (also known as Haptocorrin or Protein R) is a secreted glycoprotein that belongs to the vitamin B12-binding protein family.
• Binds vitamin B12 (cobalamin) with femtomolar affinity, facilitating its transport and protection from the acidic environment of the stomach.
• Localized to secretory granules and released into saliva and other fluids; it is a major constituent of secondary granules in neutrophils.
• Produced primarily by salivary glands in response to food ingestion, highlighting its role in early vitamin B12 handling in the digestive system.
• Contains disulfide bonds and glycosylation sites, which are important for its structural stability and function.
• Keywords: Secreted, Cobalt transport, Glycoprotein, Disulfide bond, Ion transport, Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein spanning amino acids 24-280 of human TCN1, covering most of the mature protein; this region is highly conserved, enabling cross-reactivity with mouse and rat orthologs.
• For Western blotting, a band at approximately 48kDa is expected; however, post-translational modifications may cause slight shifts. Antibody dilution should be optimized by the end user.
• In ELISA, the polyclonal nature provides robust detection but may require careful blocking to reduce background. Consider using the antibody in sandwich ELISA formats with paired detection antibodies for vitamin B12-binding studies.
• Since TCN1 is secreted, sample preparation from biological fluids (e.g., saliva, plasma) may require appropriate concentration or purification steps to achieve detectable signals in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with a streamlined supply chain for IVD raw materials. From validated antibody pairs and monoclonal/polyclonal antibodies to ancillary reagents, we support your vitamin B12 metabolism and gastrointestinal disorder research with reliable sourcing solutions. Contact us to customize bulk orders or to source rare targets like TCN1 – from concept to clinic.