Anti-TRAM1 Rabbit Polyclonal Antibody for WB - Q15629

Core Product Specifications and Parameters

Biological Background: TRAM1 Function and Localization

• Involved in the translocation of nascent protein chains into or through the endoplasmic reticulum (ER) membrane by facilitating proper chain positioning at the SEC61 channel. Related references: PMID:12475939, PMID:1315422, PMID:32013668
• Regulates the exposure of nascent secretory protein chains to the cytosol during translocation into the ER, potentially preventing premature folding. Related references: PMID:9506517
• May affect the phospholipid bilayer in the vicinity of the SEC61 lateral gate, thereby facilitating ER protein transport. Related references: PMID:32013668
• Intimately associates with transmembrane domains of nascent membrane proteins throughout the integration process and specifically binds to the second TM domain of the G protein-coupled receptor opsin/OPSD to assist its membrane insertion. Related references: PMID:8616892, PMID:12475939
• Under ER stress, participates in the unfolded protein response (UPR) by selectively retrotranslocating misfolded ER membrane proteins to the cytosol for ubiquitination and proteasomal degradation. Related references: PMID:20430023
• During cytomegalovirus infection, exploits ER-associated degradation (ERAD) to retrotranslocate MHC class I heavy chains, facilitating immune evasion.
• Localizes to the endoplasmic reticulum membrane.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal sequence (aa 305–374) of human TRAM1; consider validating detection of endogenous full-length and potential isoform-specific signals in your sample system.
• For Western blot, a band near 43 kDa is expected; however, due to glycosylation or other modifications, the observed molecular weight may shift. Optimize antibody dilution and sample preparation conditions.
• Cross-reactivity with mouse makes this antibody suitable for studies using mouse models, but always verify performance in the species of interest.
• As a polyclonal antibody, batch-to-batch consistency may vary; include appropriate positive and negative controls and titrate for new lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, research labs, and institutes with one-stop access to IVD raw materials, technical services, and expert consulting—from concept to clinic. For TRAM1-related research in ER translocation, UPR, and viral host dynamics, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw material sourcing. Let CamelBio be your reliable procurement partner for accelerating assay development and supply chain needs.