Anti-TRAFD1 Rabbit Polyclonal Antibody for WB, ELISA - O14545

Core Product Specifications and Parameters

Biological Background: TRAFD1 Function and Localization

• TRAFD1 (FLN29) is a TRAF-type zinc finger domain-containing protein encoded by the TRAFD1 gene (Gene ID: 10906) in humans.
• It acts as a negative feedback regulator that controls excessive innate immune responses by modulating TLR4 and RLH pathways.
• The protein directly interacts with TRAF6, inhibiting TLR4-mediated NF-kappa-B activation (By similarity).
• In the RLH pathway, TRAFD1 negatively regulates signaling downstream of MAVS and upstream of NF-kappa-B and IRF3 (By similarity).
• Zinc finger motifs enable metal binding and are critical for protein structure and function; TRAFD1 is also subject to acetylation and phosphorylation.
• Alternative splicing generates multiple isoforms, expanding the functional repertoire of TRAFD1 in immune regulation.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to the N-terminal region (aa 1-260) of human TRAFD1, which includes part of the zinc finger domain; thus, the antibody may recognize multiple isoforms sharing this region.
• For Western blot (WB), a band at approximately 65 kDa is expected in human samples; consider using positive controls such as immune-stimulated cell lysates where TRAFD1 expression may be upregulated.
• In ELISA, the antibody can be used for direct or indirect detection of recombinant or endogenous TRAFD1; validate coating and detection conditions for your specific assay format.
• As a polyclonal antibody, lot-to-lot variability may occur; we recommend performing internal validation with appropriate reference standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, bridging diagnostic development from research to clinical application. Our TRAFD1 Rabbit pAb, targeting the negative regulator of innate immunity, supports studies in TLR4/RLH signaling and inflammation. We offer validated antibodies, bulk reagents, and customized sourcing to meet your project needs.